Publication

VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases

Borggrewe, M., Grit, C., Den Dunnen, W. F. A., Burm, S. M., Bajramovic, J. J., Noelle, R. J., Eggen, B. J. L. & Laman, J. D., Dec-2018, In : Glia. 66, 12, p. 2645-2658 14 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Borggrewe, M., Grit, C., Den Dunnen, W. F. A., Burm, S. M., Bajramovic, J. J., Noelle, R. J., ... Laman, J. D. (2018). VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases. Glia, 66(12), 2645-2658. https://doi.org/10.1002/glia.23517

Author

Borggrewe, Malte ; Grit, Corien ; Den Dunnen, Wilfred F A ; Burm, Saskia M ; Bajramovic, Jeffrey J ; Noelle, Randolph J ; Eggen, Bart J L ; Laman, Jon D. / VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases. In: Glia. 2018 ; Vol. 66, No. 12. pp. 2645-2658.

Harvard

Borggrewe, M, Grit, C, Den Dunnen, WFA, Burm, SM, Bajramovic, JJ, Noelle, RJ, Eggen, BJL & Laman, JD 2018, 'VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases', Glia, vol. 66, no. 12, pp. 2645-2658. https://doi.org/10.1002/glia.23517

Standard

VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases. / Borggrewe, Malte; Grit, Corien; Den Dunnen, Wilfred F A; Burm, Saskia M; Bajramovic, Jeffrey J; Noelle, Randolph J; Eggen, Bart J L; Laman, Jon D.

In: Glia, Vol. 66, No. 12, 12.2018, p. 2645-2658.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Borggrewe M, Grit C, Den Dunnen WFA, Burm SM, Bajramovic JJ, Noelle RJ et al. VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases. Glia. 2018 Dec;66(12):2645-2658. https://doi.org/10.1002/glia.23517


BibTeX

@article{d85e04743793494ab3e64a56cd892e9e,
title = "VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases",
abstract = "V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) is a negative checkpoint regulator (NCR) involved in inhibition of T cell-mediated immunity. Expression changes of other NCRs (PD-1, PD-L1/L2, CTLA-4) during inflammation of the central nervous system (CNS) were previously demonstrated, but VISTA expression in the CNS has not yet been explored. Here, we report that in the human and mouse CNS, VISTA is most abundantly expressed by microglia, and to lower levels by endothelial cells. Upon TLR stimulation, VISTA expression was reduced in primary neonatal mouse and adult rhesus macaque microglia in vitro. In mice, microglial VISTA expression was reduced after lipopolysaccharide (LPS) injection, during experimental autoimmune encephalomyelitis (EAE), and in the accelerated aging Ercc1 Δ/- mouse model. After LPS injection, decreased VISTA expression in mouse microglia was accompanied by decreased acetylation of lysine residue 27 in histone 3 in both its promoter and enhancer region. ATAC-sequencing indicated a potential regulation of VISTA expression by Pu.1 and Mafb, two transcription factors crucial for microglia function. Finally, our data suggested that VISTA expression was decreased in microglia in multiple sclerosis lesion tissue, whereas it was increased in Alzheimer's disease patients. This study is the first to demonstrate that in the CNS, VISTA is expressed by microglia, and that VISTA is differentially expressed in CNS pathologies.",
keywords = "autoimmunity, cancer, DD1alpha, immune checkpoints, immunotherapy, neurodegeneration, PD-1H, IMMUNE-CHECKPOINT BLOCKADE, MULTIPLE-SCLEROSIS, AMYLOID-BETA, CELLS, MOUSE, PATHWAY, ACTIVATION, PHENOTYPE, PATHOLOGY, RESPONSES",
author = "Malte Borggrewe and Corien Grit and {Den Dunnen}, {Wilfred F A} and Burm, {Saskia M} and Bajramovic, {Jeffrey J} and Noelle, {Randolph J} and Eggen, {Bart J L} and Laman, {Jon D}",
note = "{\circledC} 2018 The Authors. Glia published by Wiley Periodicals, Inc.",
year = "2018",
month = "12",
doi = "10.1002/glia.23517",
language = "English",
volume = "66",
pages = "2645--2658",
journal = "Glia",
issn = "0894-1491",
publisher = "Wiley",
number = "12",

}

RIS

TY - JOUR

T1 - VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases

AU - Borggrewe, Malte

AU - Grit, Corien

AU - Den Dunnen, Wilfred F A

AU - Burm, Saskia M

AU - Bajramovic, Jeffrey J

AU - Noelle, Randolph J

AU - Eggen, Bart J L

AU - Laman, Jon D

N1 - © 2018 The Authors. Glia published by Wiley Periodicals, Inc.

PY - 2018/12

Y1 - 2018/12

N2 - V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) is a negative checkpoint regulator (NCR) involved in inhibition of T cell-mediated immunity. Expression changes of other NCRs (PD-1, PD-L1/L2, CTLA-4) during inflammation of the central nervous system (CNS) were previously demonstrated, but VISTA expression in the CNS has not yet been explored. Here, we report that in the human and mouse CNS, VISTA is most abundantly expressed by microglia, and to lower levels by endothelial cells. Upon TLR stimulation, VISTA expression was reduced in primary neonatal mouse and adult rhesus macaque microglia in vitro. In mice, microglial VISTA expression was reduced after lipopolysaccharide (LPS) injection, during experimental autoimmune encephalomyelitis (EAE), and in the accelerated aging Ercc1 Δ/- mouse model. After LPS injection, decreased VISTA expression in mouse microglia was accompanied by decreased acetylation of lysine residue 27 in histone 3 in both its promoter and enhancer region. ATAC-sequencing indicated a potential regulation of VISTA expression by Pu.1 and Mafb, two transcription factors crucial for microglia function. Finally, our data suggested that VISTA expression was decreased in microglia in multiple sclerosis lesion tissue, whereas it was increased in Alzheimer's disease patients. This study is the first to demonstrate that in the CNS, VISTA is expressed by microglia, and that VISTA is differentially expressed in CNS pathologies.

AB - V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) is a negative checkpoint regulator (NCR) involved in inhibition of T cell-mediated immunity. Expression changes of other NCRs (PD-1, PD-L1/L2, CTLA-4) during inflammation of the central nervous system (CNS) were previously demonstrated, but VISTA expression in the CNS has not yet been explored. Here, we report that in the human and mouse CNS, VISTA is most abundantly expressed by microglia, and to lower levels by endothelial cells. Upon TLR stimulation, VISTA expression was reduced in primary neonatal mouse and adult rhesus macaque microglia in vitro. In mice, microglial VISTA expression was reduced after lipopolysaccharide (LPS) injection, during experimental autoimmune encephalomyelitis (EAE), and in the accelerated aging Ercc1 Δ/- mouse model. After LPS injection, decreased VISTA expression in mouse microglia was accompanied by decreased acetylation of lysine residue 27 in histone 3 in both its promoter and enhancer region. ATAC-sequencing indicated a potential regulation of VISTA expression by Pu.1 and Mafb, two transcription factors crucial for microglia function. Finally, our data suggested that VISTA expression was decreased in microglia in multiple sclerosis lesion tissue, whereas it was increased in Alzheimer's disease patients. This study is the first to demonstrate that in the CNS, VISTA is expressed by microglia, and that VISTA is differentially expressed in CNS pathologies.

KW - autoimmunity

KW - cancer

KW - DD1alpha

KW - immune checkpoints

KW - immunotherapy

KW - neurodegeneration

KW - PD-1H

KW - IMMUNE-CHECKPOINT BLOCKADE

KW - MULTIPLE-SCLEROSIS

KW - AMYLOID-BETA

KW - CELLS

KW - MOUSE

KW - PATHWAY

KW - ACTIVATION

KW - PHENOTYPE

KW - PATHOLOGY

KW - RESPONSES

U2 - 10.1002/glia.23517

DO - 10.1002/glia.23517

M3 - Article

C2 - 30306644

VL - 66

SP - 2645

EP - 2658

JO - Glia

JF - Glia

SN - 0894-1491

IS - 12

ER -

ID: 66760411