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Vectorial secretion of interleukin-8 mediates autocrine signalling in intestinal epithelial cells via apically located CXCR1

Rossi, O., Karczewski, J., Stolte, E. H., Brummer, R. J. M., van Nieuwenhoven, M. A., Meijerink, M., van Neerven, J. R. J., van Ijzendoorn, S. C. D., van Baarlen, P. & Wells, J. M., 2013, In : BMC Research Notes. 6, 9 p., 341.

Research output: Contribution to journalArticleAcademicpeer-review

  • Oriana Rossi
  • Jurgen Karczewski
  • Ellen H Stolte
  • Robert J M Brummer
  • Michiel A van Nieuwenhoven
  • Marjolein Meijerink
  • Joost R J van Neerven
  • Sven C D van Ijzendoorn
  • Peter van Baarlen
  • Jerry M Wells

BACKGROUND: In the intestinal mucosa, several adaptations of TLR signalling have evolved to avoid chronic inflammatory responses to the presence of commensal microbes. Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus.

RESULTS: In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling.

CONCLUSIONS: These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo, suggesting an autocrine function for IL-8 secreted in the lumen.

Original languageEnglish
Article number341
Number of pages9
JournalBMC Research Notes
Volume6
Publication statusPublished - 2013

    Keywords

  • Autocrine Communication, Caco-2 Cells, Cell Polarity, Cells, Cultured, Gene Expression Regulation, Humans, Interleukin-8, Intestine, Small, Lipopeptides, Protein Interaction Mapping, Receptors, Interleukin-8A, Toll-Like Receptor 2, Toll-Like Receptor 5

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