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UV-inducible DNA exchange in hyperthermophilic archaea mediated by type IV pili

Ajon, M., Froels, S., van Wolferen, M., Stoecker, K., Teichmann, D., Driessen, A. J. M., Grogan, D. W., Albers, S-V., Schleper, C. & Ajon, M., Nov-2011, In : Molecular Microbiology. 82, 4, p. 807-817 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Malgorzata Ajon
  • Sabrina Froels
  • Marleen van Wolferen
  • Kilian Stoecker
  • Daniela Teichmann
  • Arnold J. M. Driessen
  • Dennis W. Grogan
  • Sonja-Verena Albers
  • Christa Schleper
  • Małgorzata Ajon

Archaea, like bacteria and eukaryotes, contain proteins involved in various mechanisms of DNA repair, highlighting the importance of these processes for all forms of life. Species of the order Sulfolobales of hyperthermophilic crenarchaeota are equipped with a strongly UV-inducible type IV pilus system that promotes cellular aggregation. Here we demonstrate by fluorescence in situ hybridization that cellular aggregates are formed based on a species-specific recognition process and that UV-induced cellular aggregation mediates chromosomal marker exchange with high frequency. Recombination rates exceeded those of uninduced cultures by up to three orders of magnitude. Knockout strains of Sulfolobus acidocaldarius incapable of pilus production could not self-aggregate, but were partners in mating experiments with wild-type strains indicating that one cellular partner can mediate the DNA transfer. Since pilus knockout strains showed decreased survival upon UV treatment, we conclude that the UV-inducible DNA transfer process and subsequent homologous recombination represents an important mechanism to maintain chromosome integrity in Sulfolobus. It might also contribute substantially to the frequent chromosomal DNA exchange and horizontal gene transfer in these archaea in their natural habitat.

Original languageEnglish
Pages (from-to)807-817
Number of pages11
JournalMolecular Microbiology
Volume82
Issue number4
Publication statusPublished - Nov-2011

    Keywords

  • EXTREMELY HIGH-TEMPERATURES, SULFOLOBUS-ACIDOCALDARIUS, HOMOLOGOUS RECOMBINATION, INTRAGENIC RECOMBINATION, SPONTANEOUS MUTATION, GENETIC EXCHANGE, SOLFATARICUS, IRRADIATION, PLASMID, REPAIR

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