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Utilization of Glycosyltransferases for the Synthesis of a Densely Packed Hyperbranched Polysaccharide Brush Coating as Artificial Glycocalyx

van der Vlist, J., Schonen, I., Loos, K. & Schönen, I., Oct-2011, In : Biomacromolecules. 12, 10, p. 3728-3732 5 p.

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  • Jeroen van der Vlist
  • Iris Schonen
  • Katja Loos
  • Iris Schönen

Densely packed polysaccharide brushes consisting of alpha-D-glucose residues were grafted from modified silicon substrates. Potato phosphorylase was herein used to grow linear polysaccharide chains from silicon tethered maltoheptaose oligosaccharides using glucose-1-phosphate as donor substrate. The combined use of potato phosphorylase and Deinococcus geothermalis branching enzyme resulted in a hyperbranched brush coating as the latter one redistributes short oligosaccharides from the alpha(1-4)-linked position to the alpha(1-6)-linked position in the polysaccharide brush. The obtained grafting density of the brushes was estimated on 1.89 nm(-2) while the thickness was measured with ellipsometric techniques and, determined to be between 12.2 and 20.2 nm.

Original languageEnglish
Pages (from-to)3728-3732
Number of pages5
JournalBiomacromolecules
Volume12
Issue number10
Publication statusPublished - Oct-2011

    Keywords

  • POLY(ETHYLENE OXIDE) BRUSHES, SELECTIVE REDUCING AGENT, ENDOTHELIAL GLYCOCALYX, THERMAL-DEGRADATION, SURFACTANT POLYMERS, PROTEIN ADSORPTION, PLATELET-ADHESION, COPOLYMERS, PREVENTION, HEPARIN

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