Utilization of Glycosyltransferases for the Synthesis of a Densely Packed Hyperbranched Polysaccharide Brush Coating as Artificial Glycocalyxvan der Vlist, J., Schonen, I., Loos, K. & Schönen, I., Oct-2011, In : Biomacromolecules. 12, 10, p. 3728-3732 5 p.
Research output: Contribution to journal › Article › Academic › peer-review
Densely packed polysaccharide brushes consisting of alpha-D-glucose residues were grafted from modified silicon substrates. Potato phosphorylase was herein used to grow linear polysaccharide chains from silicon tethered maltoheptaose oligosaccharides using glucose-1-phosphate as donor substrate. The combined use of potato phosphorylase and Deinococcus geothermalis branching enzyme resulted in a hyperbranched brush coating as the latter one redistributes short oligosaccharides from the alpha(1-4)-linked position to the alpha(1-6)-linked position in the polysaccharide brush. The obtained grafting density of the brushes was estimated on 1.89 nm(-2) while the thickness was measured with ellipsometric techniques and, determined to be between 12.2 and 20.2 nm.
|Number of pages||5|
|Publication status||Published - Oct-2011|
- POLY(ETHYLENE OXIDE) BRUSHES, SELECTIVE REDUCING AGENT, ENDOTHELIAL GLYCOCALYX, THERMAL-DEGRADATION, SURFACTANT POLYMERS, PROTEIN ADSORPTION, PLATELET-ADHESION, COPOLYMERS, PREVENTION, HEPARIN