Publication

Utilisation of Jatropha press cake as substrate in biomass and lipase production from Aspergillus niger 65I6 and Rhizomucor miehei CBS 360.62

Ilmi, M., Hidayat, C., Hastuti, P., Heeres, H. J. & van der Maarel, M. J. E. C., Jan-2017, In : Biocatalysis and Agricultural Biotechnology. 9, p. 103-107 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • Utilisation of Jatropha press cake as substrate in biomass and lipaseproduction fromAspergillus niger65I6 andRhizomucor mieheiCBS360.6

    Final publisher's version, 964 KB, PDF-document

    Request copy

DOI

Jatropha curcas L. is a tropical plant that grows well on poor soils in arid regions and produces oil-rich, unedible seeds. The oil from these seeds can be used in the production of biodiesel. In addition, the press-cake waste that is left after oil extraction contains approximately 40% of protein. The use of Jatropha press-cake as substrate for fungal growth and the production of lipase by Aspergillus niger 65I6 isolated from Indonesia and Rhizomucor miehei CBS 260.62 was investigated. Hydrolysis of the press-cake using 2.5% sodium hydroxide for 45 min was found to be the best pre-treatment method indicated by a high lipase activity. The alkaline hydrolysis increased the amount of reducing sugars and soluble protein to 133.3% and 40% of the initial amount, respectively. 5.7% of the protein fraction was hydrolysed by the alkaline pretreatment. The alkaline hydrolysed Jatropha press-cake was used as a growth substrate for A. niger 65I6 and R. miehei CBS 360.62. Both fungi grew well on the hydrolysed press cake. The addition of glucose or maltodextrin gave a substantial increase in fungal biomass but inhibited the lipase production, very likely due to catabolite repression by the sugars.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalBiocatalysis and Agricultural Biotechnology
Volume9
Publication statusPublished - Jan-2017

    Keywords

  • Plant biomass, Biodiesel, Jatropha press-cake, Lipase, Rhizomucor miehei, Aspergillus niger, SOLID-STATE FERMENTATION, BIOTECHNOLOGICAL ADVANTAGES, SUBMERGED FERMENTATION, BIODIESEL PRODUCTION, SEED CAKE, CURCAS, CELLULASE, TRANSESTERIFICATION, INDONESIA, RESIDUES

View graph of relations

ID: 77032535