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UHPLC-MS/MS method for iohexol determination in human EDTA and lithium-heparin plasma, human urine and in goat- and pig EDTA plasma

Stevens, J., Wessels, M. A., Roggeveld, J., Koster, R. A., Dekkers, C. C., van Gelder, M. K., Gansevoort, R. T., Heerspink, H. J. & Touw, D. J., 22-Jul-2020, In : Bioanalysis. 12, 14, p. 981-990 10 p.

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  • UHPLC–MS/MS method for iohexol determination in human EDTA and lithium-heparin plasma, human urine and in goat- and pig EDTA plasma

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DOI

Aim: Iohexol plasma clearance is used as an indicator of kidney function in clinical and preclinical settings. To investigate the pharmacokinetic profile of iohexol, a rapid, simple method for measurement of iohexol in different matrices and species was needed. Materials & methods: Iohexol was separated on an Accucore C18 column (Thermo Fisher Scientific, CA, USA). Detection was performed on a Thermo Scientific Quantiva tandem quadrupole mass spectrometer. The method was validated according to the requirements for bioanalytical methods issued by the US FDA and European Medicines Agency. Conclusion: We developed and validated a fast and efficient analytical method, suitable for analyzing iohexol in human EDTA plasma, human lithium-heparin plasma, human urine and goat- and pig EDTA plasma, using only one calibration line prepared in human EDTA plasma.

Original languageEnglish
Pages (from-to)981-990
Number of pages10
JournalBioanalysis
Volume12
Issue number14
Publication statusE-pub ahead of print - 22-Jul-2020

ID: 130016068