Publication

Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles

Ejby, M., Guskov, A., Pichler, M. J., Zanten, G. C., Schoof, E., Saburi, W., Slotboom, D. J. & Abou Hachem, M., Jul-2019, In : Molecular Microbiology. 112, 1, p. 114-130 17 p., 14257.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Ejby, M., Guskov, A., Pichler, M. J., Zanten, G. C., Schoof, E., Saburi, W., ... Abou Hachem, M. (2019). Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles. Molecular Microbiology, 112(1), 114-130. [14257]. https://doi.org/10.1111/mmi.14257

Author

Ejby, M ; Guskov, A ; Pichler, M J ; Zanten, G C ; Schoof, E ; Saburi, W ; Slotboom, D J ; Abou Hachem, M. / Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles. In: Molecular Microbiology. 2019 ; Vol. 112, No. 1. pp. 114-130.

Harvard

Ejby, M, Guskov, A, Pichler, MJ, Zanten, GC, Schoof, E, Saburi, W, Slotboom, DJ & Abou Hachem, M 2019, 'Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles', Molecular Microbiology, vol. 112, no. 1, 14257, pp. 114-130. https://doi.org/10.1111/mmi.14257

Standard

Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles. / Ejby, M; Guskov, A; Pichler, M J; Zanten, G C; Schoof, E; Saburi, W; Slotboom, D J; Abou Hachem, M.

In: Molecular Microbiology, Vol. 112, No. 1, 14257, 07.2019, p. 114-130.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Ejby M, Guskov A, Pichler MJ, Zanten GC, Schoof E, Saburi W et al. Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles. Molecular Microbiology. 2019 Jul;112(1):114-130. 14257. https://doi.org/10.1111/mmi.14257


BibTeX

@article{48e406da84cd4dfd96737c7689f6242f,
title = "Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles",
abstract = "Human gut bifidobacteria rely on ATP-binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide-specific solute binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize BlMnBP1 and BlMnBP2, the two SBPs associated to the β-manno-oligosaccharide (MnOS) ABC transporter in Bifidobacterium animalis subsp. lactis. Despite similar overall specificity and preference to mannotriose (Kd ≈80 nM), affinity of BlMnBP1 is up to 2570-fold higher for disaccharides than BlMnBP2. Structural analysis revealed a substitution of an asparagine that recognizes the mannosyl at position 2 in BlMnBP1, by a glycine in BlMnBP2, which affects substrate affinity. Both substitution-types occur in bifidobacterial SBPs, but BlMnBP1-like variants prevail in human-gut isolates. B. animalis subsp. lactis ATCC27673 showed growth on gluco- and galactomannans and was able to outcompete a mannan-degrading Bacteroides ovatus strain in co-cultures, attesting the efficiency of this ABC uptake system. By contrast, a strain that lacks this transporter failed to grow on mannan. This study highlights SBP diversification as a possible strategy to modulate oligosaccharide uptake preferences of bifidobacterial ABC-transporters during adaptation to specific ecological niches. Efficient metabolism of galactomannan by distinct bifidobacteria, merits evaluating this plant glycan as a potential prebiotic. This article is protected by copyright. All rights reserved.",
keywords = "GUT MICROBIOTA, RUMINOCOCCUS-ALBUS, GUAR GUM, PHOSPHORYLASES, BUTYRATE, STRAIN",
author = "M Ejby and A Guskov and Pichler, {M J} and Zanten, {G C} and E Schoof and W Saburi and Slotboom, {D J} and {Abou Hachem}, M",
note = "This article is protected by copyright. All rights reserved.",
year = "2019",
month = "7",
doi = "10.1111/mmi.14257",
language = "English",
volume = "112",
pages = "114--130",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley",
number = "1",

}

RIS

TY - JOUR

T1 - Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β-mannan possess distinct manno-oligosaccharide binding profiles

AU - Ejby, M

AU - Guskov, A

AU - Pichler, M J

AU - Zanten, G C

AU - Schoof, E

AU - Saburi, W

AU - Slotboom, D J

AU - Abou Hachem, M

N1 - This article is protected by copyright. All rights reserved.

PY - 2019/7

Y1 - 2019/7

N2 - Human gut bifidobacteria rely on ATP-binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide-specific solute binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize BlMnBP1 and BlMnBP2, the two SBPs associated to the β-manno-oligosaccharide (MnOS) ABC transporter in Bifidobacterium animalis subsp. lactis. Despite similar overall specificity and preference to mannotriose (Kd ≈80 nM), affinity of BlMnBP1 is up to 2570-fold higher for disaccharides than BlMnBP2. Structural analysis revealed a substitution of an asparagine that recognizes the mannosyl at position 2 in BlMnBP1, by a glycine in BlMnBP2, which affects substrate affinity. Both substitution-types occur in bifidobacterial SBPs, but BlMnBP1-like variants prevail in human-gut isolates. B. animalis subsp. lactis ATCC27673 showed growth on gluco- and galactomannans and was able to outcompete a mannan-degrading Bacteroides ovatus strain in co-cultures, attesting the efficiency of this ABC uptake system. By contrast, a strain that lacks this transporter failed to grow on mannan. This study highlights SBP diversification as a possible strategy to modulate oligosaccharide uptake preferences of bifidobacterial ABC-transporters during adaptation to specific ecological niches. Efficient metabolism of galactomannan by distinct bifidobacteria, merits evaluating this plant glycan as a potential prebiotic. This article is protected by copyright. All rights reserved.

AB - Human gut bifidobacteria rely on ATP-binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide-specific solute binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize BlMnBP1 and BlMnBP2, the two SBPs associated to the β-manno-oligosaccharide (MnOS) ABC transporter in Bifidobacterium animalis subsp. lactis. Despite similar overall specificity and preference to mannotriose (Kd ≈80 nM), affinity of BlMnBP1 is up to 2570-fold higher for disaccharides than BlMnBP2. Structural analysis revealed a substitution of an asparagine that recognizes the mannosyl at position 2 in BlMnBP1, by a glycine in BlMnBP2, which affects substrate affinity. Both substitution-types occur in bifidobacterial SBPs, but BlMnBP1-like variants prevail in human-gut isolates. B. animalis subsp. lactis ATCC27673 showed growth on gluco- and galactomannans and was able to outcompete a mannan-degrading Bacteroides ovatus strain in co-cultures, attesting the efficiency of this ABC uptake system. By contrast, a strain that lacks this transporter failed to grow on mannan. This study highlights SBP diversification as a possible strategy to modulate oligosaccharide uptake preferences of bifidobacterial ABC-transporters during adaptation to specific ecological niches. Efficient metabolism of galactomannan by distinct bifidobacteria, merits evaluating this plant glycan as a potential prebiotic. This article is protected by copyright. All rights reserved.

KW - GUT MICROBIOTA

KW - RUMINOCOCCUS-ALBUS

KW - GUAR GUM

KW - PHOSPHORYLASES

KW - BUTYRATE

KW - STRAIN

U2 - 10.1111/mmi.14257

DO - 10.1111/mmi.14257

M3 - Article

VL - 112

SP - 114

EP - 130

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 1

M1 - 14257

ER -

ID: 79393345