Two AAA family peroxins, PpPex1p and PpPex6p, interact with each other in an ATP-dependent manner and are associated with different subcellular membranous structures distinct from peroxisomesFaber, KN., Heyman, J. A. & Subramani, S., Feb-1998, In : Molecular and Cellular Biology. 18, 2, p. 936-943 8 p.
Research output: Contribution to journal › Article › Academic › peer-review
Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris, Cells from the corresponding deletion strains (Pp Delta pex1 and Pp Delta pex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the cytosol, and these cells cannot grow in peroxisome-requiring media (J.A. Heyman, E. Monosov, and S. Subramani, J. Cell Biol, 127:1259-1273, 1994; A. P. Spong and S, Subramani, J, Cell Biol. 123:535-548, 1993), We demonstrate that PpPex1p and PpPex6p interact in an ATP-dependent manner, Genetically, the interaction was observed in a suppressor screen with a strain harboring a temperature-sensitive allele of PpPEX1 and in the yeast two-hybrid system, Biochemially, these proteins were coimmunoprecipitated with antibodies raised against either of the proteins, but only in the presence of ATP. The protein complex formed under these conditions was 320 to 400 kDa in size, consistent with the formation of a heterodimeric PpPex1p-PpPex6p complex, Subcellular fractionation revealed PpPex1p and PpPex6p to he predominantly associated with membranous subcellular structures distinct from peroxisomes. Based on their behavior in subcellular fractionation experiments including flotation gradients and on the fact that these structures are also present in a Pp Delta ex3 strain in which no morphologically detectable proxisomal remnants have been observed, we propose that these structures are small vesicles, The identification of vesicle-associated peroxins is novel and implies a role for these vesicles in peroxisome biogenesis. We discuss the possible role of the ATP-dependent interaction between PpPex1p and PpPex6p in regulating peroxisome biogenesis events.
|Number of pages||8|
|Journal||Molecular and Cellular Biology|
|Publication status||Published - Feb-1998|
- YEAST PICHIA-PASTORIS, PROTEIN IMPORT DEFICIENCIES, HANSENULA-POLYMORPHA, SACCHAROMYCES-CEREVISIAE, PUTATIVE ATPASES, TARGETING SIGNAL, BIOGENESIS, GENE, ENCODES, FUSION