Publication

TRANSLOCATION OF FLUORESCENT ETHER PHOSPHOLIPID, BUT NOT ITS DIACYL COUNTERPART, AFTER INSERTION IN PLASMA-MEMBRANES OF CONTROL AND PLASMALOGEN-DEFICIENT FIBROBLASTS

VANDERVEER, E., VANDERWEIDE, D., HEIJMANS, HSA. & HOEKSTRA, D., 14-Mar-1993, In : Biochimica et biophysica acta. 1146, 2, p. 294-300 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Fluorescently labelled ether phospholipid (1-0-alkyl/alkenyl-2-acyl-glycerophosphocholine) readily internalizes at low temperatures (2-degrees-C) after insertion into the plasma membrane of cultured fibroblasts. This fate differs markedly from that of its diacyl phospholipid analogue, which remains associated with the plasma membrane under similar conditions. Analysis by thin-layer chromatography reveals that the translocation involves transfer of the intact ether phosphatidylcholine molecules. Relative to control cells, a 2-fold increase of ether phosphatidylcholine uptake was noted when plasmalogen deficient fibroblasts were used. Back-exchange experiments demonstrate that more than 60% of the cell-associated ether lipid is translocated within the cells, irrespective of the cell strain that was used. The potential mechanism by which the translocation process is accomplished is discussed.

Original languageEnglish
Pages (from-to)294-300
Number of pages7
JournalBiochimica et biophysica acta
Volume1146
Issue number2
Publication statusPublished - 14-Mar-1993

    Keywords

  • ETHER GLYCEROPHOSPHOLIPID, TRANSLOCATION, PLASMALOGEN-DEFICIENT FIBROBLAST, FIBROBLAST, HUMAN-SKIN FIBROBLASTS, ZELLWEGER SYNDROME, ENDOCYTIC PATHWAY, BIOSYNTHESIS, PEROXISOMES, ANALOG, CELLS

ID: 6344280