Publication

Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano

Grudniewska, M., Mouton, S., Simanov, D., Beltman, F., Grelling, M., de Mulder, K., Arindrarto, W., Weissert, P. M., van der Elst, S. & Berezikov, E., 20-Dec-2016, In : eLife. 5, 23 p., 20607.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Grudniewska, M., Mouton, S., Simanov, D., Beltman, F., Grelling, M., de Mulder, K., Arindrarto, W., Weissert, P. M., van der Elst, S., & Berezikov, E. (2016). Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano. eLife, 5, [20607]. https://doi.org/10.7554/eLife.20607

Author

Grudniewska, Magda ; Mouton, Stijn ; Simanov, Daniil ; Beltman, Frank ; Grelling, Margriet ; de Mulder, Katrien ; Arindrarto, Wibowo ; Weissert, Philipp M. ; van der Elst, Stefan ; Berezikov, Eugene. / Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano. In: eLife. 2016 ; Vol. 5.

Harvard

Grudniewska, M, Mouton, S, Simanov, D, Beltman, F, Grelling, M, de Mulder, K, Arindrarto, W, Weissert, PM, van der Elst, S & Berezikov, E 2016, 'Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano', eLife, vol. 5, 20607. https://doi.org/10.7554/eLife.20607

Standard

Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano. / Grudniewska, Magda; Mouton, Stijn; Simanov, Daniil; Beltman, Frank; Grelling, Margriet; de Mulder, Katrien; Arindrarto, Wibowo; Weissert, Philipp M.; van der Elst, Stefan; Berezikov, Eugene.

In: eLife, Vol. 5, 20607, 20.12.2016.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Grudniewska M, Mouton S, Simanov D, Beltman F, Grelling M, de Mulder K et al. Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano. eLife. 2016 Dec 20;5. 20607. https://doi.org/10.7554/eLife.20607


BibTeX

@article{e43ba069e45f4956ad22a938245239b6,
title = "Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano",
abstract = "he regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.",
keywords = "PLANARIAN SCHMIDTEA-MEDITERRANEA, PLURIPOTENT STEM-CELLS, RNA-SEQ DATA, GENE-EXPRESSION, FLUORESCENT SPERM, TRANSPARENT WORM, SEXUAL SELECTION, REGENERATION, FLATWORM, GENOME",
author = "Magda Grudniewska and Stijn Mouton and Daniil Simanov and Frank Beltman and Margriet Grelling and {de Mulder}, Katrien and Wibowo Arindrarto and Weissert, {Philipp M.} and {van der Elst}, Stefan and Eugene Berezikov",
year = "2016",
month = dec,
day = "20",
doi = "10.7554/eLife.20607",
language = "English",
volume = "5",
journal = "eLife",
issn = "2050-084X",
publisher = "ELIFE SCIENCES PUBLICATIONS LTD",

}

RIS

TY - JOUR

T1 - Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano

AU - Grudniewska, Magda

AU - Mouton, Stijn

AU - Simanov, Daniil

AU - Beltman, Frank

AU - Grelling, Margriet

AU - de Mulder, Katrien

AU - Arindrarto, Wibowo

AU - Weissert, Philipp M.

AU - van der Elst, Stefan

AU - Berezikov, Eugene

PY - 2016/12/20

Y1 - 2016/12/20

N2 - he regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.

AB - he regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As a flatworm amenable to transgenesis, it complements the historically used planarian flatworm models, such as Schmidtea mediterranea. However, information on the transcriptome and markers of stem cells in M. lignano is limited. We generated a de novo transcriptome assembly and performed the first comprehensive characterization of gene expression in the proliferating cells of M. lignano, represented by somatic stem cells, called neoblasts, and germline cells. Knockdown of a selected set of neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, and Mlig-h2a, confirmed their crucial role for the functionality of somatic neoblasts during homeostasis and regeneration. The generated M. lignano transcriptome assembly and gene expression signatures of somatic neoblasts and germline cells will be a valuable resource for future molecular studies in M. lignano.

KW - PLANARIAN SCHMIDTEA-MEDITERRANEA

KW - PLURIPOTENT STEM-CELLS

KW - RNA-SEQ DATA

KW - GENE-EXPRESSION

KW - FLUORESCENT SPERM

KW - TRANSPARENT WORM

KW - SEXUAL SELECTION

KW - REGENERATION

KW - FLATWORM

KW - GENOME

U2 - 10.7554/eLife.20607

DO - 10.7554/eLife.20607

M3 - Article

VL - 5

JO - eLife

JF - eLife

SN - 2050-084X

M1 - 20607

ER -

ID: 39534231