Publication

Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha

Leao-Helder, A. N., Krikken, A. M., van der Klei, I. J., Kiel, J. A. K. W. & Veenhuis, M., 17-Oct-2003, In : The Journal of Biological Chemistry. 278, 42, p. 40749-40756 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Leao-Helder, A. N., Krikken, A. M., van der Klei, I. J., Kiel, J. A. K. W., & Veenhuis, M. (2003). Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha. The Journal of Biological Chemistry, 278(42), 40749-40756. https://doi.org/10.1074/jbc.M304029200

Author

Leao-Helder, Adriana Nívea ; Krikken, Arjen M ; van der Klei, Ida J ; Kiel, Jan A K W ; Veenhuis, Marten. / Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha. In: The Journal of Biological Chemistry. 2003 ; Vol. 278, No. 42. pp. 40749-40756.

Harvard

Leao-Helder, AN, Krikken, AM, van der Klei, IJ, Kiel, JAKW & Veenhuis, M 2003, 'Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha', The Journal of Biological Chemistry, vol. 278, no. 42, pp. 40749-40756. https://doi.org/10.1074/jbc.M304029200

Standard

Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha. / Leao-Helder, Adriana Nívea; Krikken, Arjen M; van der Klei, Ida J; Kiel, Jan A K W; Veenhuis, Marten.

In: The Journal of Biological Chemistry, Vol. 278, No. 42, 17.10.2003, p. 40749-40756.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Leao-Helder AN, Krikken AM, van der Klei IJ, Kiel JAKW, Veenhuis M. Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha. The Journal of Biological Chemistry. 2003 Oct 17;278(42):40749-40756. https://doi.org/10.1074/jbc.M304029200


BibTeX

@article{62444434eab14d5fa976213f11216747,
title = "Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha",
abstract = "We have isolated and characterized a novel transcription factor of Hansenula polymorpha that is involved in the regulation of peroxisomal protein levels. This protein, designated Mpp1p, belongs to the family of Zn(II)(2)Cys(6) proteins. In cells deleted for the function of Mpp1p the levels of various proteins involved in peroxisome biogenesis (peroxins) and function ( enzymes) are reduced compared with wild type or, in the case of the matrix protein dihydroxyacetone synthase, fully absent. Also, upon induction of mpp1 cells on methanol, the number of peroxisomes was strongly reduced relative to wild type cells and generally amounted to one organelle per cell. Remarkably, this single organelle was not susceptible to selective peroxisome degradation (pexophagy) and remained unaffected during exposure of methanol-induced cells to excess glucose conditions. We show that this mechanism is a general phenomenon in H. polymorpha in the case of cells that contain only a single peroxisome.",
keywords = "Aldehyde-Ketone Transferases, Amino Acid Sequence, Cloning, Molecular, DNA, Down-Regulation, Glucose, Green Fluorescent Proteins, Luminescent Proteins, Methanol, Molecular Sequence Data, Mutagenesis, Mutation, Peroxisomes, Pichia, Recombinant Fusion Proteins, Sequence Homology, Amino Acid, Time Factors, Transcription Factors, Transcription, Genetic, Zinc, Zinc Fingers",
author = "Leao-Helder, {Adriana N{\'i}vea} and Krikken, {Arjen M} and {van der Klei}, {Ida J} and Kiel, {Jan A K W} and Marten Veenhuis",
year = "2003",
month = oct,
day = "17",
doi = "10.1074/jbc.M304029200",
language = "English",
volume = "278",
pages = "40749--40756",
journal = "The Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC",
number = "42",

}

RIS

TY - JOUR

T1 - Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha

AU - Leao-Helder, Adriana Nívea

AU - Krikken, Arjen M

AU - van der Klei, Ida J

AU - Kiel, Jan A K W

AU - Veenhuis, Marten

PY - 2003/10/17

Y1 - 2003/10/17

N2 - We have isolated and characterized a novel transcription factor of Hansenula polymorpha that is involved in the regulation of peroxisomal protein levels. This protein, designated Mpp1p, belongs to the family of Zn(II)(2)Cys(6) proteins. In cells deleted for the function of Mpp1p the levels of various proteins involved in peroxisome biogenesis (peroxins) and function ( enzymes) are reduced compared with wild type or, in the case of the matrix protein dihydroxyacetone synthase, fully absent. Also, upon induction of mpp1 cells on methanol, the number of peroxisomes was strongly reduced relative to wild type cells and generally amounted to one organelle per cell. Remarkably, this single organelle was not susceptible to selective peroxisome degradation (pexophagy) and remained unaffected during exposure of methanol-induced cells to excess glucose conditions. We show that this mechanism is a general phenomenon in H. polymorpha in the case of cells that contain only a single peroxisome.

AB - We have isolated and characterized a novel transcription factor of Hansenula polymorpha that is involved in the regulation of peroxisomal protein levels. This protein, designated Mpp1p, belongs to the family of Zn(II)(2)Cys(6) proteins. In cells deleted for the function of Mpp1p the levels of various proteins involved in peroxisome biogenesis (peroxins) and function ( enzymes) are reduced compared with wild type or, in the case of the matrix protein dihydroxyacetone synthase, fully absent. Also, upon induction of mpp1 cells on methanol, the number of peroxisomes was strongly reduced relative to wild type cells and generally amounted to one organelle per cell. Remarkably, this single organelle was not susceptible to selective peroxisome degradation (pexophagy) and remained unaffected during exposure of methanol-induced cells to excess glucose conditions. We show that this mechanism is a general phenomenon in H. polymorpha in the case of cells that contain only a single peroxisome.

KW - Aldehyde-Ketone Transferases

KW - Amino Acid Sequence

KW - Cloning, Molecular

KW - DNA

KW - Down-Regulation

KW - Glucose

KW - Green Fluorescent Proteins

KW - Luminescent Proteins

KW - Methanol

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Mutation

KW - Peroxisomes

KW - Pichia

KW - Recombinant Fusion Proteins

KW - Sequence Homology, Amino Acid

KW - Time Factors

KW - Transcription Factors

KW - Transcription, Genetic

KW - Zinc

KW - Zinc Fingers

U2 - 10.1074/jbc.M304029200

DO - 10.1074/jbc.M304029200

M3 - Article

C2 - 12902346

VL - 278

SP - 40749

EP - 40756

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

SN - 0021-9258

IS - 42

ER -

ID: 4135132