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Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha
Leao-Helder, A. N., Krikken, A. M., van der Klei, I. J., Kiel, J. A. K. W. & Veenhuis, M., 17-Oct-2003, In : The Journal of Biological Chemistry. 278, 42, p. 40749-40756 8 p.Research output: Contribution to journal › Article › Academic › peer-review
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Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha. / Leao-Helder, Adriana Nívea; Krikken, Arjen M; van der Klei, Ida J; Kiel, Jan A K W; Veenhuis, Marten.
In: The Journal of Biological Chemistry, Vol. 278, No. 42, 17.10.2003, p. 40749-40756.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - Transcriptional down-regulation of peroxisome numbers affects selective peroxisome degradation in Hansenula polymorpha
AU - Leao-Helder, Adriana Nívea
AU - Krikken, Arjen M
AU - van der Klei, Ida J
AU - Kiel, Jan A K W
AU - Veenhuis, Marten
PY - 2003/10/17
Y1 - 2003/10/17
N2 - We have isolated and characterized a novel transcription factor of Hansenula polymorpha that is involved in the regulation of peroxisomal protein levels. This protein, designated Mpp1p, belongs to the family of Zn(II)(2)Cys(6) proteins. In cells deleted for the function of Mpp1p the levels of various proteins involved in peroxisome biogenesis (peroxins) and function ( enzymes) are reduced compared with wild type or, in the case of the matrix protein dihydroxyacetone synthase, fully absent. Also, upon induction of mpp1 cells on methanol, the number of peroxisomes was strongly reduced relative to wild type cells and generally amounted to one organelle per cell. Remarkably, this single organelle was not susceptible to selective peroxisome degradation (pexophagy) and remained unaffected during exposure of methanol-induced cells to excess glucose conditions. We show that this mechanism is a general phenomenon in H. polymorpha in the case of cells that contain only a single peroxisome.
AB - We have isolated and characterized a novel transcription factor of Hansenula polymorpha that is involved in the regulation of peroxisomal protein levels. This protein, designated Mpp1p, belongs to the family of Zn(II)(2)Cys(6) proteins. In cells deleted for the function of Mpp1p the levels of various proteins involved in peroxisome biogenesis (peroxins) and function ( enzymes) are reduced compared with wild type or, in the case of the matrix protein dihydroxyacetone synthase, fully absent. Also, upon induction of mpp1 cells on methanol, the number of peroxisomes was strongly reduced relative to wild type cells and generally amounted to one organelle per cell. Remarkably, this single organelle was not susceptible to selective peroxisome degradation (pexophagy) and remained unaffected during exposure of methanol-induced cells to excess glucose conditions. We show that this mechanism is a general phenomenon in H. polymorpha in the case of cells that contain only a single peroxisome.
KW - Aldehyde-Ketone Transferases
KW - Amino Acid Sequence
KW - Cloning, Molecular
KW - DNA
KW - Down-Regulation
KW - Glucose
KW - Green Fluorescent Proteins
KW - Luminescent Proteins
KW - Methanol
KW - Molecular Sequence Data
KW - Mutagenesis
KW - Mutation
KW - Peroxisomes
KW - Pichia
KW - Recombinant Fusion Proteins
KW - Sequence Homology, Amino Acid
KW - Time Factors
KW - Transcription Factors
KW - Transcription, Genetic
KW - Zinc
KW - Zinc Fingers
U2 - 10.1074/jbc.M304029200
DO - 10.1074/jbc.M304029200
M3 - Article
C2 - 12902346
VL - 278
SP - 40749
EP - 40756
JO - The Journal of Biological Chemistry
JF - The Journal of Biological Chemistry
SN - 0021-9258
IS - 42
ER -
ID: 4135132