Towards the development of activity-based probes for detection of lysine-specific demethylase-1 activityOurailidou, M. E., Lenoci, A., Zwergel, C., Rotili, D., Mai, A. & Dekker, F. J., 1-Feb-2017, In : Bioorganic & Medicinal Chemistry. 25, 3, p. 847-856 10 p.
Research output: Contribution to journal › Article › Academic › peer-review
The implications of lysine-specific demethylase-1 (LSD1) in tumorigenesis have urged scientists to develop diagnostic tools in order to explore the function of this enzyme. In this work, we present our efforts on the development of tranylcypromine (TCP)-based functionalized probes for activity-based protein profiling (ABPP) of LSD1 activity. Biotinylated forms of selected compounds enabled dose-dependent enzyme labeling of recombinant LSD1. However, treatment with LSD1 inhibitors did not clearly reduce the LSD1 labeling efficiency thus indicating that labeling using these probes is not activity dependent. This calls for alternative strategies to develop probes for ABPP of the enzyme LSD1.
|Number of pages||10|
|Journal||Bioorganic & Medicinal Chemistry|
|Publication status||Published - 1-Feb-2017|
- Lysine demethylation, Tumorigenesis, Tranylcypromine, Irreversible inhibition, Enzyme labeling, LSD1 HISTONE DEMETHYLASE, OXIDATIVE HECK REACTION, THERAPEUTIC STRATEGY, BIOLOGICAL-ACTIVITY, STRUCTURAL BASIS, HDAC INHIBITORS, BREAST-CANCER, PROTEIN, CELLS, DERIVATIVES