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Toward dynamic structural biology: Two decades of single-molecule Forster resonance energy transfer

Lerner, E., Cordes, T., Ingargiola, A., Alhadid, Y., Chung, S., Michalet, X. & Weiss, S., 19-Jan-2018, In : Science. 359, 6373, 13 p., 1133.

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  • Toward dynamic structural biology:Two decades of single-moleculeFörster resonance energy transfer

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DOI

  • Eitan Lerner
  • Thorben Cordes
  • Antonino Ingargiola
  • Yazan Alhadid
  • SangYoon Chung
  • Xavier Michalet
  • Shimon Weiss

Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Forster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.

Original languageEnglish
Article number1133
Number of pages13
JournalScience
Volume359
Issue number6373
Publication statusPublished - 19-Jan-2018

    Keywords

  • ALTERNATING-LASER EXCITATION, PROTEIN CONFORMATIONAL DYNAMICS, X-RAY CRYSTALLOGRAPHY, FLUORESCENCE SPECTROSCOPY, RNA-POLYMERASE, FRET EXPERIMENTS, LIVING CELLS, QUANTITATIVE FRET, ELECTRON-TRANSFER, FOLDING DYNAMICS

ID: 77227207