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Topologically fixed SecG is fully functional

Sluis, E. O. V. D., van der Vries, E., Berrelkamp, G., Nouwen, N. & Driessen, AJM., Feb-2006, In : Journal of Bacteriology. 188, 3, p. 1188-1190 3 p.

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DOI

It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.

Original languageEnglish
Pages (from-to)1188-1190
Number of pages3
JournalJournal of Bacteriology
Volume188
Issue number3
Publication statusPublished - Feb-2006

    Keywords

  • PROTEIN TRANSLOCATION MACHINERY, ESCHERICHIA-COLI, CROSS-LINKING, CYTOPLASMIC MEMBRANE, PREPROTEIN, COMPLEX, INVERSION, COMPONENT, SUBUNITS, ROLES

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