Topologically fixed SecG is fully functionalSluis, E. O. V. D., van der Vries, E., Berrelkamp, G., Nouwen, N. & Driessen, AJM., Feb-2006, In : Journal of Bacteriology. 188, 3, p. 1188-1190 3 p.
Research output: Contribution to journal › Article › Academic › peer-review
It has been proposed that the bitopic membrane protein SecG undergoes topology inversion during translocation of (pre)proteins via SecYEG. Here we show that SecG covalently cross-linked to SecY cannot invert its topology while remaining fully functional in protein translocation. Our results strongly disfavor topology inversion of SecG during protein translocation.
|Number of pages||3|
|Journal||Journal of Bacteriology|
|Publication status||Published - Feb-2006|
- PROTEIN TRANSLOCATION MACHINERY, ESCHERICHIA-COLI, CROSS-LINKING, CYTOPLASMIC MEMBRANE, PREPROTEIN, COMPLEX, INVERSION, COMPONENT, SUBUNITS, ROLES