Topography-driven alterations in endothelial cell phenotype and contact guidance

Suarez, A. M. A., van der Ham, I., Brinker, M. G. L., van Rijn, P. & Harmsen, M. C., Jun-2020, In : Heliyon. 6, 6, 13 p., 04329.

Research output: Contribution to journalArticleAcademicpeer-review

Understanding how endothelial cell phenotype is affected by topography could improve the design of new tools for tissue engineering as many tissue engineering approaches make use of topography-mediated cell stimulation. Therefore, we cultured human pulmonary microvascular endothelial cells (ECs) on a directional topographical gradient to screen the EC vascular-like network formation and alignment response to nano to microsized to- pographies. The cell response was evaluated by microscopy. We found that ECs formed unstable vascular-like networks that aggregated in the smaller topographies and flat parts whereas ECs themselves aligned on the larger topographies. Subsequently, we designed a mixed topography where we could explore the network for- mation and proliferative properties of these ECs by live imaging for three days. Vascular-like network formation continued to be unstable on the topography and were only produced on the flat areas and a fibronectin coating did not improve the network stability. However, an instructive adipose tissue-derived stromal cell (ASC) coating provided the correct environment to sustain the vascular-like networks, which were still affected by the topog- raphy underneath. It was concluded that large microsized topographies inhibit vascular endothelial network formation but not proliferation and flat and nano/microsized topographies allow formation of early networks that can be stabilized by using an ASC instructive layer.

Original languageEnglish
Article number04329
Number of pages13
Issue number6
Publication statusPublished - Jun-2020


  • Bioengineering, Biophysics, Cell biology, Biomedical engineering, Regenerative medicine, Directional topography, Endothelial cells, Vascular-like networks, Contact guidance, Vascularization, STROMAL CELLS, IN-VITRO, MIGRATION, ANGIOGENESIS

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