Tools for genetic engineering of the yeast Hansenula polymorphaSaraya, R., Gidijala, L., Veenhuis, M. & van der Klei, I. J., 2014, Yeast Metabolic Engineering: Methods and Protocols. Mapelli, V. (ed.). Springer Verlag, Vol. 1152. p. 43-62 20 p. (Springer Protocols; vol. 1152).
Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic
Hansenula polymorpha is a methylotrophic yeast species that has favorable properties for heterologous protein production and metabolic engineering. It provides an attractive expression platform with the capability to secrete high levels of commercially important proteins. Over the past few years many efforts have led to advances in the development of this microbial host including the generation of expression vectors containing strong constitutive or inducible promoters and a large array of dominant and auxotrophic markers. Moreover, highly efficient transformation procedures used to generate genetically stable strains are now available. Here, we describe these tools as well as the methods for genetic engineering of H. polymorpha.
|Title of host publication||Yeast Metabolic Engineering|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||20|
|Publication status||Published - 2014|
- Genetic engineering, Hansenula polymorpha, Methylotrophic yeast, Metabolic engineering, Heterologous protein production