Publication

Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates

Kiewiet, M. B. G., Dekkers, R., Gros, M., van Neerven, R. J. J., Groeneveld, A., de Vos, P. & Faas, M. M. 8-Jun-2017 In : PLoS ONE. 12, 6, 17 p., 0178191

Research output: Scientific - peer-reviewArticle

APA

Kiewiet, M. B. G., Dekkers, R., Gros, M., van Neerven, R. J. J., Groeneveld, A., de Vos, P., & Faas, M. M. (2017). Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates. PLoS ONE, 12(6), [0178191]. DOI: 10.1371/journal.pone.0178191

Author

Kiewiet, M. B. Gea ; Dekkers, Renske ; Gros, Marjan ; van Neerven, R. J. Joost ; Groeneveld, Andre ; de Vos, Paul ; Faas, Marijke M./ Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates. In: PLoS ONE. 2017 ; Vol. 12, No. 6.

Harvard

Kiewiet, MBG, Dekkers, R, Gros, M, van Neerven, RJJ, Groeneveld, A, de Vos, P & Faas, MM 2017, 'Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates' PLoS ONE, vol 12, no. 6, 0178191. DOI: 10.1371/journal.pone.0178191

Standard

Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates. / Kiewiet, M. B. Gea; Dekkers, Renske; Gros, Marjan; van Neerven, R. J. Joost; Groeneveld, Andre; de Vos, Paul; Faas, Marijke M.

In: PLoS ONE, Vol. 12, No. 6, 0178191, 08.06.2017.

Research output: Scientific - peer-reviewArticle

Vancouver

Kiewiet MBG, Dekkers R, Gros M, van Neerven RJJ, Groeneveld A, de Vos P et al. Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates. PLoS ONE. 2017 Jun 8;12(6). 0178191. Available from, DOI: 10.1371/journal.pone.0178191


BibTeX

@article{fcff9effcc864477a84d5ecee0b777a8,
title = "Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates",
abstract = "Immunomodulating proteins and peptides are formed during the hydrolysis of cow's milk proteins. These proteins are potential ingredients in functional foods used for the management of a range of immune related problems, both in infants and adults. However, the mechanism behind these effects is unknown. We hypothesize that the interaction of peptides with Toll-like receptors (TLRs) can induce immune effects, since these receptors are known to sample many dietary molecules in the intestine in order to regulate immune effects. To investigate this, we compared the immune effects and TLR activation and inhibition by whey and casein hydrolysates with different hydrolysis levels. We first measured cytokine production in primary peripheral blood mononuclear cells stimulated with either whey, casein, or their hydrolysates. IL-10 and TNF alpha were induced by whey hydrolysates (decreasing with increasing hydrolysis level), but not by casein hydrolysates. Next, the activation of TLR 2, 3, 5 and 9 receptors were observed by intact and mildly hydrolysed whey proteins only and not by casein hydrolysates in TLR reporter cell lines. Many casein hydrolysates inhibited TLR signaling (mainly TLR 5 and 9). These results demonstrate that the effects of cow's milk proteins on the immune system are protein type and hydrolysis dependent. TLR signaling is suggested as a possible mechanism for differences in effect. This knowledge contributes to a better understanding of the immune effects of hydrolysates and the design of infant formula, and nutrition in general, with specific immunoregulatory effects.",
keywords = "BOVINE BETA-CASEIN, PATTERN-RECOGNITION RECEPTORS, LIMULUS AMEBOCYTE LYSATE, PROTEIN HYDROLYSATE, BIOACTIVE PEPTIDES, IN-VITRO, ENHANCES PROLIFERATION, IMMUNE-RESPONSES, EPITHELIAL-CELLS, IL-8 SECRETION",
author = "Kiewiet, {M. B. Gea} and Renske Dekkers and Marjan Gros and {van Neerven}, {R. J. Joost} and Andre Groeneveld and {de Vos}, Paul and Faas, {Marijke M.}",
year = "2017",
month = "6",
doi = "10.1371/journal.pone.0178191",
volume = "12",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "6",

}

RIS

TY - JOUR

T1 - Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow's milk hydrolysates

AU - Kiewiet,M. B. Gea

AU - Dekkers,Renske

AU - Gros,Marjan

AU - van Neerven,R. J. Joost

AU - Groeneveld,Andre

AU - de Vos,Paul

AU - Faas,Marijke M.

PY - 2017/6/8

Y1 - 2017/6/8

N2 - Immunomodulating proteins and peptides are formed during the hydrolysis of cow's milk proteins. These proteins are potential ingredients in functional foods used for the management of a range of immune related problems, both in infants and adults. However, the mechanism behind these effects is unknown. We hypothesize that the interaction of peptides with Toll-like receptors (TLRs) can induce immune effects, since these receptors are known to sample many dietary molecules in the intestine in order to regulate immune effects. To investigate this, we compared the immune effects and TLR activation and inhibition by whey and casein hydrolysates with different hydrolysis levels. We first measured cytokine production in primary peripheral blood mononuclear cells stimulated with either whey, casein, or their hydrolysates. IL-10 and TNF alpha were induced by whey hydrolysates (decreasing with increasing hydrolysis level), but not by casein hydrolysates. Next, the activation of TLR 2, 3, 5 and 9 receptors were observed by intact and mildly hydrolysed whey proteins only and not by casein hydrolysates in TLR reporter cell lines. Many casein hydrolysates inhibited TLR signaling (mainly TLR 5 and 9). These results demonstrate that the effects of cow's milk proteins on the immune system are protein type and hydrolysis dependent. TLR signaling is suggested as a possible mechanism for differences in effect. This knowledge contributes to a better understanding of the immune effects of hydrolysates and the design of infant formula, and nutrition in general, with specific immunoregulatory effects.

AB - Immunomodulating proteins and peptides are formed during the hydrolysis of cow's milk proteins. These proteins are potential ingredients in functional foods used for the management of a range of immune related problems, both in infants and adults. However, the mechanism behind these effects is unknown. We hypothesize that the interaction of peptides with Toll-like receptors (TLRs) can induce immune effects, since these receptors are known to sample many dietary molecules in the intestine in order to regulate immune effects. To investigate this, we compared the immune effects and TLR activation and inhibition by whey and casein hydrolysates with different hydrolysis levels. We first measured cytokine production in primary peripheral blood mononuclear cells stimulated with either whey, casein, or their hydrolysates. IL-10 and TNF alpha were induced by whey hydrolysates (decreasing with increasing hydrolysis level), but not by casein hydrolysates. Next, the activation of TLR 2, 3, 5 and 9 receptors were observed by intact and mildly hydrolysed whey proteins only and not by casein hydrolysates in TLR reporter cell lines. Many casein hydrolysates inhibited TLR signaling (mainly TLR 5 and 9). These results demonstrate that the effects of cow's milk proteins on the immune system are protein type and hydrolysis dependent. TLR signaling is suggested as a possible mechanism for differences in effect. This knowledge contributes to a better understanding of the immune effects of hydrolysates and the design of infant formula, and nutrition in general, with specific immunoregulatory effects.

KW - BOVINE BETA-CASEIN

KW - PATTERN-RECOGNITION RECEPTORS

KW - LIMULUS AMEBOCYTE LYSATE

KW - PROTEIN HYDROLYSATE

KW - BIOACTIVE PEPTIDES

KW - IN-VITRO

KW - ENHANCES PROLIFERATION

KW - IMMUNE-RESPONSES

KW - EPITHELIAL-CELLS

KW - IL-8 SECRETION

U2 - 10.1371/journal.pone.0178191

DO - 10.1371/journal.pone.0178191

M3 - Article

VL - 12

JO - PLoS ONE

T2 - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 6

M1 - 0178191

ER -

ID: 42761176