Publication

Timing of erythropoietin treatment for cardioprotection in ischemia/reperfusion

Lipsic, E., van der Meer, P., Henning, RH., Suurmeijer, AJH., Boddeus, KM., van Veldhuisen, DJ., van Gilst, WH. & Schoemaker, RG., Oct-2004, In : Journal of Cardiovascular Pharmacology. 44, 4, p. 473-479 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Erythropoietin (EPO) is a hormone known to stimulate hematopoiesis. However, recent research suggests additional properties of EPO, such as protection against ischemia/reperfusion (I/R) injury in various tissues. We studied the effect of timing of EPO administration on cardioprotection during I/R in the heart. Male Sprague-Dawley rats were subjected to 45 minutes of coronary occlusion, followed by 24 hours of reperfusion. Animals were randomized to receive saline or single dose of EPO (5000 IU/kg) either 2 hours before I/R, at the start of ischemia, or after the onset of reperfusion. The ratio of infarct area/area at risk (planimetry), left ventricular (LV) function (pressure development), and apoptosis (number of active caspase-3 positive cells) were determined after 24-hour reperfusion. Administration of EPO during different time points resulted in a 19 to 23% (P <0.05) reduction in the infarct area/area at risk, which was accompanied by a trend toward better LV hemodynamic parameters. Apoptosis was significantly attenuated in groups treated with EPO at the start of ischemia (29% reduction) and after the onset of reperfusion (38%), and to a lesser extent (16%) in the group pre-treated with EPO. Thus, in vivo administration of EPO at different time points protects the myocardial structure and preserves cardiac function during I/R. Cardioprotective effect of EPO is associated with inhibition of apoptosis.

Original languageEnglish
Pages (from-to)473-479
Number of pages7
JournalJournal of Cardiovascular Pharmacology
Volume44
Issue number4
Publication statusPublished - Oct-2004

    Keywords

  • erythropoietin, ischemia, reperfusion, apoptosis, ISCHEMIA-REPERFUSION INJURY, RECOMBINANT-HUMAN-ERYTHROPOIETIN, MYOCARDIAL-INFARCTION, ENDOTHELIAL-CELLS, BRAIN-INJURY, NITRIC-OXIDE, IN-VIVO, APOPTOSIS, HEART, PROTECTS

ID: 4265765