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The unprocessed preprotein form IAT(C103S) of the isopenicillin N acyltransferase is transported inside peroxisomes and regulates its self-processing

Garcia-Estrada, C., Vaca, I., Fierro, F., Sjollema, K., Veenhuis, M. & Francisco Martin, J., Jun-2008, In : Fungal Genetics and Biology. 45, 6, p. 1043-1052 10 p.

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  • The unprocessed preprotein form IATC103S

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DOI

  • Carlos Garcia-Estrada
  • Inmaculada Vaca
  • Francisco Fierro
  • Klaas Sjollema
  • Marten Veenhuis
  • Juan Francisco Martin

Previous studies in Penicillium chrysogenum and Aspergillus nidulans suggested that self-processing of the isopenicillin N acyltransferase (IAT) is an important differential factor in these fungi. Expression of a mutant penDE(C103S) gene in P. chrysogenum gave rise to an unprocessed inactive variant of IAT (IAT(C103S)) located inside peroxisomes, which indicates that transport of the proIAT inside these organelles is not dependent on the processing state of the protein. Co-expression of the penDE(C103S) and wild-type penDE genes in P. chrysogenum (Wis54-DE(C103S) strain) led to a decrease in benzylpenicillin levels. Changes in the wild-type IAT processing profile (P subunit formation) were observed in the Wis54-DE(C103S) strain, suggesting a regulatory role of the unprocessed IAT(C103S) in the processing of the wild-type IAT. This was confirmed in Escherichia coli, where a delay in the processing of IAT in presence of the unprocessable IAT(C103S) was observed. Our results indicate that IAT is post-translationally regulated by its preprotein, which interferes with the self-processing. (c) 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)1043-1052
Number of pages10
JournalFungal Genetics and Biology
Volume45
Issue number6
Publication statusPublished - Jun-2008

    Keywords

  • self-processing, NTN enzyme, peroxisomes, benzylpenicillin biosynthesis, post-translational regulation, PENICILLIN GENE-CLUSTER, ACYL-COENZYME-A, COENZYME-A-6-AMINOPENICILLANIC ACID ACYLTRANSFERASE, ESCHERICHIA-COLI, ASPERGILLUS-NIDULANS, MOLECULAR CHARACTERIZATION, BIOSYNTHETIC-PATHWAY, TANDEM REPEATS, CHRYSOGENUM, ENZYME

ID: 4731110