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The stability and functional properties of proteoliposomes mixed with dextran derivatives bearing hydrophobic anchor groups

Elferink, M. G. L., Wit, J. G. D., Veld, G. I. ., Reichert, A., Driessen, A. J. M., Ringsdorf, H. & Konings, W., 29-Apr-1992, In : Biochimica et biophysica acta. 1106, 1, p. 23-30 8 p.

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  • The stability and functional properties of proteoliposomes mixed with dextran derivatives bearing hydrophobic anchor groups

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DOI

Liposomes composed of Escherichia coli phospholipid were coated with polysaccharides bearing hydrophobic palmitoyl anchors. The effect on the stability of liposomes without or with integral membrane proteins was investigated. A high concentration of hydrophobized dextrans protected the liposomes against detergent degradation, decreased the fluidity of the membranes, prevented fusion of the liposomes and enhanced their stability. Proteoliposomes containing beef heart cytochrome-c oxidase and the lactose transport carrier of E. coli were similarly affected by coating with the dextrans. Under these conditions both membrane proteins were still active. Long-term stability of the coated liposomes was obtained only in the absence of the integral membrane proteins.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalBiochimica et biophysica acta
Volume1106
Issue number1
Publication statusPublished - 29-Apr-1992

    Keywords

  • PROTEOLIPOSOME, INTEGRAL MEMBRANE PROTEIN, DEXTRAN DERIVATIVE, HYDROPHOBIC ANCHOR GROUP, PROTON-MOTIVE FORCE, BIOLOGICAL-MEMBRANES, ESCHERICHIA-COLI, CYTOCHROME-C, VESICLES, PROTEIN, KINETICS, OXIDASE, BINDING, FUSION

ID: 3331352