The role of polyglucose in oxygen-dependent respiration by a new strain of Desulfovibrio salexigens

van Niel, E. W. J., Pedro Gomes, T. M., Willems, A., Collins, M. D., Prins, R. A. & Gottschal, J. C., Dec-1996, In : FEMS Microbial Ecology. 21, 4, p. 243-253 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard


  • The role of polyglucose in oxygen-dependent respiration

    Final publisher's version, 1 MB, PDF document


  • Ed W. J. van Niel
  • Teresa M. Pedro Gomes
  • Anne Willems
  • Matthew D. Collins
  • Rudolf A. Prins
  • Jan C. Gottschal

Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8-1.0 g polyglucose (g protein)(-1)), but the growth rare with this substrate was very low (0.015 h(-1)). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose was present. Maximum oxygen reduction rates were found at air saturating concentrations and were relatively low (3-50 nmol O-2 min(-1) (mg protein)(-1)). Catalase was constitutively present in anaerobically grown cells, When batch cultures were exposed to oxygen, growth ceased immediately and polyglucose was oxidized to acetate within 40-50 h, Like the oxygen reduction activity, the nitro blue tetrazolium (NBT)-reduction activity in these cells was proportional to the polyglucose content. Under anaerobic starvation conditions there was no correlation between the NBT-reduction activity and polyglucose concentration and polyglucose was degraded slowly within 240 h, The ecological significance of aerobic polyglucose consumption is discussed.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalFEMS Microbial Ecology
Issue number4
Publication statusPublished - Dec-1996


  • Desulfovibrio salexigens, sulfate reduction, glucose, polyglucose, oxygen-dependent respiration, SULFATE-REDUCING BACTERIA, HYDROGEN TRANSFER, REDUCTION, GROWTH, DESULFURICANS, GIGAS, DESULFOBULBUS, METABOLISM, CULTURES, MATS

ID: 6492538