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The role of polyglucose in oxygen-dependent respiration by a new strain of Desulfovibrio salexigens

vanNiel, EWJ., Gomes, TMP., Willems, A., Collins, MD., Prins, RA. & Gottschal, JC., Dec-1996, In : FEMS Microbial Ecology. 21, 4, p. 243-253 11 p.

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  • EWJ vanNiel
  • TMP Gomes
  • A Willems
  • MD Collins
  • RA Prins
  • JC Gottschal

Desulfovibrio salexigens strain Mastl was isolated from the oxic/anoxic interface of a marine sediment. Growth under sulfate-reducing conditions was accompanied by polyglucose accumulation in the cell with every substrate tested. Highest polyglucose storage was found with glucose (0.8-1.0 g polyglucose (g protein)(-1)), but the growth rare with this substrate was very low (0.015 h(-1)). Anaerobically grown cells of strain Mastl exhibited immediate oxygen-dependent respiration. The endogenous oxygen reduction rate was proportional to the polyglucose content. The rate of aerobic respiration of pyruvate was also directly related to the polyglucose content indicating that this organism was only able to respire with oxygen as long as polyglucose was present. Maximum oxygen reduction rates were found at air saturating concentrations and were relatively low (3-50 nmol O-2 min(-1) (mg protein)(-1)). Catalase was constitutively present in anaerobically grown cells, When batch cultures were exposed to oxygen, growth ceased immediately and polyglucose was oxidized to acetate within 40-50 h, Like the oxygen reduction activity, the nitro blue tetrazolium (NBT)-reduction activity in these cells was proportional to the polyglucose content. Under anaerobic starvation conditions there was no correlation between the NBT-reduction activity and polyglucose concentration and polyglucose was degraded slowly within 240 h, The ecological significance of aerobic polyglucose consumption is discussed.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalFEMS Microbial Ecology
Volume21
Issue number4
Publication statusPublished - Dec-1996

    Keywords

  • Desulfovibrio salexigens, sulfate reduction, glucose, polyglucose, oxygen-dependent respiration, SULFATE-REDUCING BACTERIA, HYDROGEN TRANSFER, REDUCTION, GROWTH, DESULFURICANS, GIGAS, DESULFOBULBUS, METABOLISM, CULTURES, MATS

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