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The role of hepatocytes in the clearance of liposomes from the blood circulation

Scherphof, GL. & Kamps, JAAM., May-2001, In : Progress in lipid research. 40, 3, p. 149-166 18 p.

Research output: Contribution to journalReview articleAcademicpeer-review

In this chapter we summarize literature and describe in more detail our own observations over a period of nearly two decennia on the role of hepatocytes in the hepatic clearance of intravenously administered liposomes, Evidence is presented indicating that, although size is an important parameter, it is not decisive in determining access of liposomes to the hepatocytes. Also lipid composition is an important parameter, including charge, rigidity and headgroup composition. The role of the fenestrated sinusoidal endothelial cells in determining liposome accessibility of hepatocytes is discussed as well as the involvement of opsonizing plasma proteins such as apolipoprotein E. Our observations led us to postulate the existence of at least four different mechanisms of interaction of liposomes with hepatocytes, i.e. an endocytic and a non-endocytic one for both neutral and negatively charged vesicles (C) 2001 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)149-166
Number of pages18
JournalProgress in lipid research
Volume40
Issue number3
Publication statusPublished - May-2001

    Keywords

  • apolipoproteins, endothelial fenestrations, hepatocytes, kupffer cells, lipoprotein receptors, liposome uptake (mechanisms), liver, opsonisation, sinusoidal endothelial cells, SMALL UNILAMELLAR LIPOSOMES, ASIALOFETUIN-LABELED LIPOSOMES, HUMAN ALPHA-1-ANTITRYPSIN GENE, KUPFFER CELLS INVIVO, IN-VIVO, RAT HEPATOCYTES, MOUSE-LIVER, HEPATIC-UPTAKE, ASIALOGLYCOPROTEIN RECEPTOR, INTRAHEPATIC DISTRIBUTION

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