The role of Hansenula polymorpha MIG1 homologues in catabolite repression and pexophagyStasyk, O. G., Van Zutphen, T., Kang, H. A., Stasyk, O. V., Veenhuis, M. & Sibirny, A. A., Oct-2007, In : Fems Yeast Research. 7, 7, p. 1103-1113 11 p.
Research output: Contribution to journal › Article › Academic › peer-review
In the methanol-utilizing yeast Hansenula polymorpha, glucose and ethanol trigger the repression of peroxisomal enzymes at the transcriptional level, and rapid and selective degradation of methanol-induced peroxisomes by means of a process termed pexophagy. In this report we demonstrate that deficiency in the putative H. polymorpha homologues of transcriptional repressors Mig1 (HpMig1 and HpMig2), as well as HpTup1, partially and differentially affects the repression of peroxisomal alcohol oxidase by sugars and ethanol. As reported earlier, deficiency in HpTup1 leads to impairment of glucose- or ethanol-induced macropexophagy. In H. polymorpha mig1mig2 double-deletion cells, macropexophagy was also substantially impaired, whereas micropexophagy became a dominant mode of autophagic degradation. Our findings suggest that homologues of the elements of the Saccharomyces cerevisiae main repression pathway have pleiotropic functions in H. polymorpha.
|Number of pages||11|
|Journal||Fems Yeast Research|
|Publication status||Published - Oct-2007|
|Event||4th Hansenula Polymorpha Worldwide Network Conference (HPWN) - , Netherlands|
Duration: 3-Sep-2006 → 5-Sep-2006
4th Hansenula Polymorpha Worldwide Network Conference (HPWN)
03/09/2006 → 05/09/2006Netherlands
- yeast, Hansenula polymorpha, catabolite repression, pexophagy, transcriptional repressors, YEAST HANSENULA-POLYMORPHA, CONTROLS GLUCOSE REPRESSION, METHYLOTROPHIC YEAST, SACCHAROMYCES-CEREVISIAE, MIG1 REPRESSOR, METHANOL METABOLISM, SELECTIVE AUTOPHAGY, CANDIDA-ALBICANS, GENE ENCODES, PEROXISOMES