Publication

The Response of HeLa Cells to Fluorescent NanoDiamond Uptake

Hemelaar, S. R., Saspaanithy, B., L'Hommelet, S. R. M., Perona Martinez, F. P., van der Laan, K. J. & Schirhagl, R., 26-Jan-2018, In : Sensors. 18, 2, 15 p., 355.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Hemelaar, S. R., Saspaanithy, B., L'Hommelet, S. R. M., Perona Martinez, F. P., van der Laan, K. J., & Schirhagl, R. (2018). The Response of HeLa Cells to Fluorescent NanoDiamond Uptake. Sensors, 18(2), [355]. https://doi.org/10.3390/s18020355

Author

Hemelaar, Simon R ; Saspaanithy, Babujhi ; L'Hommelet, Severin R M ; Perona Martinez, Felipe P ; van der Laan, Kiran J ; Schirhagl, Romana. / The Response of HeLa Cells to Fluorescent NanoDiamond Uptake. In: Sensors. 2018 ; Vol. 18, No. 2.

Harvard

Hemelaar, SR, Saspaanithy, B, L'Hommelet, SRM, Perona Martinez, FP, van der Laan, KJ & Schirhagl, R 2018, 'The Response of HeLa Cells to Fluorescent NanoDiamond Uptake', Sensors, vol. 18, no. 2, 355. https://doi.org/10.3390/s18020355

Standard

The Response of HeLa Cells to Fluorescent NanoDiamond Uptake. / Hemelaar, Simon R; Saspaanithy, Babujhi; L'Hommelet, Severin R M; Perona Martinez, Felipe P; van der Laan, Kiran J; Schirhagl, Romana.

In: Sensors, Vol. 18, No. 2, 355, 26.01.2018.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Hemelaar SR, Saspaanithy B, L'Hommelet SRM, Perona Martinez FP, van der Laan KJ, Schirhagl R. The Response of HeLa Cells to Fluorescent NanoDiamond Uptake. Sensors. 2018 Jan 26;18(2). 355. https://doi.org/10.3390/s18020355


BibTeX

@article{800e9fbfc92f42f6ab27d14631515590,
title = "The Response of HeLa Cells to Fluorescent NanoDiamond Uptake",
abstract = "Fluorescent nanodiamonds are promising probes for nanoscale magnetic resonance measurements. Their physical properties predict them to have particularly useful applications in intracellular analysis. Before using them in intracellular experiments however, it should be clear whether diamond particles influence cell biology. While cytotoxicity has already been ruled out in previous studies, we consider the non-fatal influence of fluorescent nanodiamonds on the formation of reactive oxygen species (an important stress indicator and potential target for intracellular sensing) for the first time. We investigated the influence of different sizes, shapes and concentrations of nanodiamonds on the genetic and protein level involved in oxidative stress-related pathways of the HeLa cell, an important model cell line in research. The changes in viability of the cells and the difference in intracellular levels of free radicals, after diamond uptake, are surprisingly small. At lower diamond concentrations, the cellular metabolism cannot be distinguished from that of untreated cells. This research supports the claims of non-toxicity and includes less obvious non-fatal responses. Finally, we give a handhold concerning the diamond concentration and size to use for non-toxic, intracellular measurements in favour of (cancer) research in HeLa cells.",
keywords = "Journal Article, ENHANCEMENT, DETONATION, NECROSIS-FACTOR-ALPHA, IN-VIVO, DRUG-DELIVERY, FUNCTIONALIZED NANODIAMONDS, DIAMOND, SPIN, BIOCOMPATIBILITY, BIODISTRIBUTION",
author = "Hemelaar, {Simon R} and Babujhi Saspaanithy and L'Hommelet, {Severin R M} and {Perona Martinez}, {Felipe P} and {van der Laan}, {Kiran J} and Romana Schirhagl",
year = "2018",
month = "1",
day = "26",
doi = "10.3390/s18020355",
language = "English",
volume = "18",
journal = "Sensors",
issn = "1424-8220",
publisher = "MDPI AG",
number = "2",

}

RIS

TY - JOUR

T1 - The Response of HeLa Cells to Fluorescent NanoDiamond Uptake

AU - Hemelaar, Simon R

AU - Saspaanithy, Babujhi

AU - L'Hommelet, Severin R M

AU - Perona Martinez, Felipe P

AU - van der Laan, Kiran J

AU - Schirhagl, Romana

PY - 2018/1/26

Y1 - 2018/1/26

N2 - Fluorescent nanodiamonds are promising probes for nanoscale magnetic resonance measurements. Their physical properties predict them to have particularly useful applications in intracellular analysis. Before using them in intracellular experiments however, it should be clear whether diamond particles influence cell biology. While cytotoxicity has already been ruled out in previous studies, we consider the non-fatal influence of fluorescent nanodiamonds on the formation of reactive oxygen species (an important stress indicator and potential target for intracellular sensing) for the first time. We investigated the influence of different sizes, shapes and concentrations of nanodiamonds on the genetic and protein level involved in oxidative stress-related pathways of the HeLa cell, an important model cell line in research. The changes in viability of the cells and the difference in intracellular levels of free radicals, after diamond uptake, are surprisingly small. At lower diamond concentrations, the cellular metabolism cannot be distinguished from that of untreated cells. This research supports the claims of non-toxicity and includes less obvious non-fatal responses. Finally, we give a handhold concerning the diamond concentration and size to use for non-toxic, intracellular measurements in favour of (cancer) research in HeLa cells.

AB - Fluorescent nanodiamonds are promising probes for nanoscale magnetic resonance measurements. Their physical properties predict them to have particularly useful applications in intracellular analysis. Before using them in intracellular experiments however, it should be clear whether diamond particles influence cell biology. While cytotoxicity has already been ruled out in previous studies, we consider the non-fatal influence of fluorescent nanodiamonds on the formation of reactive oxygen species (an important stress indicator and potential target for intracellular sensing) for the first time. We investigated the influence of different sizes, shapes and concentrations of nanodiamonds on the genetic and protein level involved in oxidative stress-related pathways of the HeLa cell, an important model cell line in research. The changes in viability of the cells and the difference in intracellular levels of free radicals, after diamond uptake, are surprisingly small. At lower diamond concentrations, the cellular metabolism cannot be distinguished from that of untreated cells. This research supports the claims of non-toxicity and includes less obvious non-fatal responses. Finally, we give a handhold concerning the diamond concentration and size to use for non-toxic, intracellular measurements in favour of (cancer) research in HeLa cells.

KW - Journal Article

KW - ENHANCEMENT

KW - DETONATION

KW - NECROSIS-FACTOR-ALPHA

KW - IN-VIVO

KW - DRUG-DELIVERY

KW - FUNCTIONALIZED NANODIAMONDS

KW - DIAMOND

KW - SPIN

KW - BIOCOMPATIBILITY

KW - BIODISTRIBUTION

U2 - 10.3390/s18020355

DO - 10.3390/s18020355

M3 - Article

VL - 18

JO - Sensors

JF - Sensors

SN - 1424-8220

IS - 2

M1 - 355

ER -

ID: 55323087