Publication

The reduction in small ribosomal subunit abundance in ethanol-stressed cells of Bacillus subtilis is mediated by a SigB-dependent antisense RNA

Mars, R. A. T., Mendonca, K., Denham, E. L. & van Dijl, J. M., Oct-2015, In : Biochimica et Biophysica Acta - Molecular Cell Research. 1853, 10, p. 2553-2559 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

One of the best-characterized general stress responses in bacteria is the sigma(B)-mediated stress response of the Gram-positive soil bacterium Bacillus subtilis. The sigma(B) regulon contains approximately 200 protein-encoding genes and 136 putative regulatory RNAs. One of these sigma(B)-dependent RNAs, named S1136-S1134, was recently mapped as being transcribed from the S1136 promoter on the opposite strand of the essential rpsD gene, which encodes the ribosomal primary-binding protein S4. Accordingly, S1136-S1134 transcription results in an rpsD-overlapping antisense RNA (asRNA). Upon exposure of B. subtilis to ethanol, the S1136 promoter was found to be induced, while rpsD transcription was downregulated. By quantitative PCR, we show that the activation of transcription from the S1136 promoter is directly responsible for the downregulation of rpsD upon ethanol exposure. We also show that this downregulation of rpsD leads to a reduced level of the small (30S) ribosomal subunit upon ethanol stress. The activation of the S1136 promoter thus represents the first example of antisense transcription-mediated regulation in the general stress response of B. subtilis and implicates the reduction of ribosomal protein abundance as a new aspect in the sigma(B)-dependent stress response. We propose that the observed reduction in the level of the small ribosomal subunit, which contains the ribosome-decoding center, may protect B. subtilis cells against misreading and spurious translation of possibly toxic aberrant peptides under conditions of ethanol stress. (C) 2015 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)2553-2559
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1853
Issue number10
Publication statusPublished - Oct-2015

    Keywords

  • Antisense RNA, sigma(B), Stress response, Bacillus subtilis, Primary binding protein, Ribosome, TRANSCRIPTION FACTOR SIGMA(B), PROTEIN-BIOSYNTHESIS, EXPRESSION, BACTERIA, MODEL, GENE, ARCHITECTURE, ACTIVATION, ANTITOXIN, REGULON

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