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The Phosphoenolpyruvate: Sugar Phosphotransferase System Is Involved in Sensitivity to the Glucosylated Bacteriocin Sublancin

De Gonzalo, C. V. G., Denham, E. L., Mars, R. A. T., Stuelke, J., van der Donk, W. A. & van Dijl, J. M., Nov-2015, In : Antimicrobial Agents and Chemotherapy. 59, 11, p. 6844-6854 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

The mode of action of a group of glycosylated antimicrobial peptides known as glycocins remains to be elucidated. In the current study of one glycocin, sublancin, we identified the phosphoenolpyruvate: sugar phosphotransferase system (PTS) of Bacillus species as a key player in bacterial sensitivity. Sublancin kills several Gram-positive bacteria, such as Bacillus species and Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA). Unlike other classes of bacteriocins for which the PTS is involved in their mechanism of action, we show that the addition of PTS-requiring sugars leads to increased resistance rather than increased sensitivity, suggesting that sublancin has a distinct mechanism of action. Collectively, our present mutagenesis and genomic studies demonstrate that the histidine-containing phosphocarrier protein (HPr) and domain A of enzyme II (PtsG) in particular are critical determinants for bacterial sensitivity to sublancin.

Original languageEnglish
Pages (from-to)6844-6854
Number of pages11
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number11
Publication statusPublished - Nov-2015

    Keywords

  • CARBON CATABOLITE REPRESSION, S-LINKED GLYCOPEPTIDE, BACILLUS-SUBTILIS, LIPID-II, PHOSPHOCARRIER PROTEIN, STAPHYLOCOCCUS-AUREUS, RESISTANCE, EXPRESSION, GENES, HPR

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