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The Pex4p-Pex22p complex from Hansenula polymorpha: biophysical analysis, crystallization and X-ray diffraction characterization

Ali, A. M., Atmaj, J., Adawy, A., Lunev, S., Van Oosterwijk, N., Yan, S. R., Williams, C. & Groves, M. R., Feb-2018, In : Acta Crystallographica Section F: Structural Biology Communications . 74, Pt 2, p. 76-81 6 p., ISSN 2053-230X.

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  • Ameena M Ali
  • Jack Atmaj
  • Alaa Adawy
  • Sergey Lunev
  • Niels Van Oosterwijk
  • Sun Rei Yan
  • Chris Williams
  • Matthew R Groves

Peroxisomes are a major cellular compartment of eukaryotic cells, and are involved in a variety of metabolic functions and pathways according to species, cell type and environmental conditions. Their biogenesis relies on conserved genes known as PEX genes that encode peroxin proteins. Peroxisomal membrane proteins and peroxisomal matrix proteins are generated in the cytosol and are subsequently imported into the peroxisome post-translationally. Matrix proteins containing a peroxisomal targeting signal type 1 (PTS1) are recognized by the cycling receptor Pex5p and transported to the peroxisomal lumen. Pex5p docking, release of the cargo into the lumen and recycling involve a number of peroxins, but a key player is the Pex4p-Pex22p complex described in this manuscript. Pex4p from the yeast Saccharomyces cerevisiae is a ubiquitin-conjugating enzyme that is anchored on the cytosolic side of the peroxisomal membrane through its binding partner Pex22p, which acts as both a docking site and a co-activator of Pex4p. As Pex5p undergoes recycling and release, the Pex4p-Pex22p complex is essential for monoubiquitination at the conserved cysteine residue of Pex5p. The absence of Pex4p-Pex22p inhibits Pex5p recycling and hence PTS1 protein import. This article reports the crystallization of Pex4p and of the Pex4p-Pex22p complex from the yeast Hansenula polymorpha, and data collection from their crystals to 2.0 and 2.85 Å resolution, respectively. The resulting structures are likely to provide important insights to understand the molecular mechanism of the Pex4p-Pex22p complex and its role in peroxisome biogenesis.

Original languageEnglish
Article numberISSN 2053-230X
Pages (from-to)76-81
Number of pages6
JournalActa Crystallographica Section F: Structural Biology Communications
Volume74
Issue numberPt 2
Publication statusPublished - Feb-2018

    Keywords

  • Hansenula polymorpha, Pex4, Pex22, ubiquitin-conjugating enzyme, peroxisome import, IMPORT RECEPTOR PEX5P, MATRIX PROTEIN IMPORT, PEROXISOMAL MEMBRANE, UBIQUITINATION, BIOGENESIS, BIOCHEMISTRY, RECOGNITION, PATHWAYS, CYSTEINE

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