Publication

The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis

Gabarrini, G., de Smit, M., Westra, J., Brouwer, E., Vissink, A., Zhou, K., Rossen, J. W. A., Stobernack, T., van Dijl, J. M. & van Winkelhoff, A. J., 25-Sep-2015, In : Scientific Reports. 5, 8 p., 13936.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Gabarrini, G., de Smit, M., Westra, J., Brouwer, E., Vissink, A., Zhou, K., ... van Winkelhoff, A. J. (2015). The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis. Scientific Reports, 5, [13936]. https://doi.org/10.1038/srep13936

Author

Gabarrini, Giorgio ; de Smit, Menke ; Westra, Johanna ; Brouwer, Elisabeth ; Vissink, Arjan ; Zhou, Kai ; Rossen, John W. A. ; Stobernack, Tim ; van Dijl, Jan Maarten ; van Winkelhoff, Arie Jan. / The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis. In: Scientific Reports. 2015 ; Vol. 5.

Harvard

Gabarrini, G, de Smit, M, Westra, J, Brouwer, E, Vissink, A, Zhou, K, Rossen, JWA, Stobernack, T, van Dijl, JM & van Winkelhoff, AJ 2015, 'The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis' Scientific Reports, vol. 5, 13936. https://doi.org/10.1038/srep13936

Standard

The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis. / Gabarrini, Giorgio; de Smit, Menke; Westra, Johanna; Brouwer, Elisabeth; Vissink, Arjan; Zhou, Kai; Rossen, John W. A.; Stobernack, Tim; van Dijl, Jan Maarten; van Winkelhoff, Arie Jan.

In: Scientific Reports, Vol. 5, 13936, 25.09.2015.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Gabarrini G, de Smit M, Westra J, Brouwer E, Vissink A, Zhou K et al. The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis. Scientific Reports. 2015 Sep 25;5. 13936. https://doi.org/10.1038/srep13936


BibTeX

@article{43dd83a473394e659923bf61f8577f1f,
title = "The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis",
abstract = "Periodontitis is an infective process that ultimately leads to destruction of the soft and hard tissues that support the teeth (the periodontium). Periodontitis has been proposed as a candidate risk factor for development of the autoimmune disease rheumatoid arthritis (RA). Porphyromonas gingivalis, a major periodontal pathogen, is the only known prokaryote expressing a peptidyl arginine deiminase (PAD) enzyme necessary for protein citrullination. Antibodies to citrullinated proteins (anti-citrullinated protein antibodies, ACPA) are highly specific for RA and precede disease onset. Objective of this study was to assess P. gingivalis PAD (PPAD) gene expression and citrullination patterns in representative samples of P. gingivalis clinical isolates derived from periodontitis patients with and without RA and in related microbes of the Porphyromonas genus. Our findings indicate that PPAD is omnipresent in P. gingivalis, but absent in related species. No significant differences were found in the composition and expression of the PPAD gene of P. gingivalis regardless of the presence of RA or periodontal disease phenotypes. From this study it can be concluded that if P. gingivalis plays a role in RA, it is unlikely to originate from a variation in PPAD gene expression.",
keywords = "RHEUMATOID-ARTHRITIS, PERIODONTITIS, PURIFICATION, ANTIBODIES, FIBRINOGEN, ENOLASE",
author = "Giorgio Gabarrini and {de Smit}, Menke and Johanna Westra and Elisabeth Brouwer and Arjan Vissink and Kai Zhou and Rossen, {John W. A.} and Tim Stobernack and {van Dijl}, {Jan Maarten} and {van Winkelhoff}, {Arie Jan}",
year = "2015",
month = "9",
day = "25",
doi = "10.1038/srep13936",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - The peptidylarginine deiminase gene is a conserved feature of Porphyromonas gingivalis

AU - Gabarrini, Giorgio

AU - de Smit, Menke

AU - Westra, Johanna

AU - Brouwer, Elisabeth

AU - Vissink, Arjan

AU - Zhou, Kai

AU - Rossen, John W. A.

AU - Stobernack, Tim

AU - van Dijl, Jan Maarten

AU - van Winkelhoff, Arie Jan

PY - 2015/9/25

Y1 - 2015/9/25

N2 - Periodontitis is an infective process that ultimately leads to destruction of the soft and hard tissues that support the teeth (the periodontium). Periodontitis has been proposed as a candidate risk factor for development of the autoimmune disease rheumatoid arthritis (RA). Porphyromonas gingivalis, a major periodontal pathogen, is the only known prokaryote expressing a peptidyl arginine deiminase (PAD) enzyme necessary for protein citrullination. Antibodies to citrullinated proteins (anti-citrullinated protein antibodies, ACPA) are highly specific for RA and precede disease onset. Objective of this study was to assess P. gingivalis PAD (PPAD) gene expression and citrullination patterns in representative samples of P. gingivalis clinical isolates derived from periodontitis patients with and without RA and in related microbes of the Porphyromonas genus. Our findings indicate that PPAD is omnipresent in P. gingivalis, but absent in related species. No significant differences were found in the composition and expression of the PPAD gene of P. gingivalis regardless of the presence of RA or periodontal disease phenotypes. From this study it can be concluded that if P. gingivalis plays a role in RA, it is unlikely to originate from a variation in PPAD gene expression.

AB - Periodontitis is an infective process that ultimately leads to destruction of the soft and hard tissues that support the teeth (the periodontium). Periodontitis has been proposed as a candidate risk factor for development of the autoimmune disease rheumatoid arthritis (RA). Porphyromonas gingivalis, a major periodontal pathogen, is the only known prokaryote expressing a peptidyl arginine deiminase (PAD) enzyme necessary for protein citrullination. Antibodies to citrullinated proteins (anti-citrullinated protein antibodies, ACPA) are highly specific for RA and precede disease onset. Objective of this study was to assess P. gingivalis PAD (PPAD) gene expression and citrullination patterns in representative samples of P. gingivalis clinical isolates derived from periodontitis patients with and without RA and in related microbes of the Porphyromonas genus. Our findings indicate that PPAD is omnipresent in P. gingivalis, but absent in related species. No significant differences were found in the composition and expression of the PPAD gene of P. gingivalis regardless of the presence of RA or periodontal disease phenotypes. From this study it can be concluded that if P. gingivalis plays a role in RA, it is unlikely to originate from a variation in PPAD gene expression.

KW - RHEUMATOID-ARTHRITIS

KW - PERIODONTITIS

KW - PURIFICATION

KW - ANTIBODIES

KW - FIBRINOGEN

KW - ENOLASE

U2 - 10.1038/srep13936

DO - 10.1038/srep13936

M3 - Article

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 13936

ER -

ID: 24088662