Publication

The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish

Paridaen, J. T. M. L., Janson, E., Utami, K. H., Pereboom, T. C., Essers, P. B., van Rooijen, C., Zivkovic, D. & MacInnes, A. W., 15-Jul-2011, In : Developmental Biology. 355, 2, p. 286-301 16 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Paridaen, J. T. M. L., Janson, E., Utami, K. H., Pereboom, T. C., Essers, P. B., van Rooijen, C., ... MacInnes, A. W. (2011). The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish. Developmental Biology, 355(2), 286-301. https://doi.org/10.1016/j.ydbio.2011.04.028

Author

Paridaen, Judith T M L ; Janson, Esther ; Utami, Kagistia Hana ; Pereboom, Tamara C ; Essers, Paul B ; van Rooijen, Carina ; Zivkovic, Danica ; MacInnes, Alyson W. / The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish. In: Developmental Biology. 2011 ; Vol. 355, No. 2. pp. 286-301.

Harvard

Paridaen, JTML, Janson, E, Utami, KH, Pereboom, TC, Essers, PB, van Rooijen, C, Zivkovic, D & MacInnes, AW 2011, 'The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish' Developmental Biology, vol. 355, no. 2, pp. 286-301. https://doi.org/10.1016/j.ydbio.2011.04.028

Standard

The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish. / Paridaen, Judith T M L; Janson, Esther; Utami, Kagistia Hana; Pereboom, Tamara C; Essers, Paul B; van Rooijen, Carina; Zivkovic, Danica; MacInnes, Alyson W.

In: Developmental Biology, Vol. 355, No. 2, 15.07.2011, p. 286-301.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Paridaen JTML, Janson E, Utami KH, Pereboom TC, Essers PB, van Rooijen C et al. The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish. Developmental Biology. 2011 Jul 15;355(2):286-301. https://doi.org/10.1016/j.ydbio.2011.04.028


BibTeX

@article{329b099315774ee8add33253e3a8e1ec,
title = "The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish",
abstract = "Nucleostemin (NS), a member of a family of nucleolar GTP-binding proteins, is highly expressed in proliferating cells such as stem and cancer cells and is involved in the control of cell cycle progression. Both depletion and overexpression of NS result in stabilization of the tumor suppressor p53 protein in vitro. Although it has been previously suggested that NS has p53-independent functions, these to date remain unknown. Here, we report two zebrafish mutants recovered from forward and reverse genetic screens that carry loss of function mutations in two members of this nucleolar protein family, Guanine nucleotide binding-protein-like 2 (Gnl2) and Gnl3/NS. We demonstrate that these proteins are required for correct timing of cell cycle exit and subsequent neural differentiation in the brain and retina. Concomitantly, we observe aberrant expression of the cell cycle regulators cyclinD1 and p57kip2. Our models demonstrate that the loss of Gnl2 or NS induces p53 stabilization and p53-mediated apoptosis. However, the retinal differentiation defects are independent of p53 activation. Furthermore, this work demonstrates that Gnl2 and NS have both non-cell autonomously and cell-autonomous function in correct timing of cell cycle exit and neural differentiation. Finally, the data suggest that Gnl2 and NS affect cell cycle exit of neural progenitors by regulating the expression of cell cycle regulators independently of p53.",
keywords = "Animals, Blotting, Western, Bromodeoxyuridine, Cell Cycle, Cyclin D1, Cyclin-Dependent Kinase Inhibitor p57, GTP-Binding Proteins, Gene Expression Regulation, Immunohistochemistry, In Situ Hybridization, Microarray Analysis, Microscopy, Fluorescence, Mutation, Neurogenesis, Nuclear Proteins, Oligonucleotides, Plasmids, Retina, Zebrafish, Journal Article, Research Support, Non-U.S. Gov't",
author = "Paridaen, {Judith T M L} and Esther Janson and Utami, {Kagistia Hana} and Pereboom, {Tamara C} and Essers, {Paul B} and {van Rooijen}, Carina and Danica Zivkovic and MacInnes, {Alyson W}",
note = "Copyright {\circledC} 2011 Elsevier Inc. All rights reserved.",
year = "2011",
month = "7",
day = "15",
doi = "10.1016/j.ydbio.2011.04.028",
language = "English",
volume = "355",
pages = "286--301",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "2",

}

RIS

TY - JOUR

T1 - The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish

AU - Paridaen, Judith T M L

AU - Janson, Esther

AU - Utami, Kagistia Hana

AU - Pereboom, Tamara C

AU - Essers, Paul B

AU - van Rooijen, Carina

AU - Zivkovic, Danica

AU - MacInnes, Alyson W

N1 - Copyright © 2011 Elsevier Inc. All rights reserved.

PY - 2011/7/15

Y1 - 2011/7/15

N2 - Nucleostemin (NS), a member of a family of nucleolar GTP-binding proteins, is highly expressed in proliferating cells such as stem and cancer cells and is involved in the control of cell cycle progression. Both depletion and overexpression of NS result in stabilization of the tumor suppressor p53 protein in vitro. Although it has been previously suggested that NS has p53-independent functions, these to date remain unknown. Here, we report two zebrafish mutants recovered from forward and reverse genetic screens that carry loss of function mutations in two members of this nucleolar protein family, Guanine nucleotide binding-protein-like 2 (Gnl2) and Gnl3/NS. We demonstrate that these proteins are required for correct timing of cell cycle exit and subsequent neural differentiation in the brain and retina. Concomitantly, we observe aberrant expression of the cell cycle regulators cyclinD1 and p57kip2. Our models demonstrate that the loss of Gnl2 or NS induces p53 stabilization and p53-mediated apoptosis. However, the retinal differentiation defects are independent of p53 activation. Furthermore, this work demonstrates that Gnl2 and NS have both non-cell autonomously and cell-autonomous function in correct timing of cell cycle exit and neural differentiation. Finally, the data suggest that Gnl2 and NS affect cell cycle exit of neural progenitors by regulating the expression of cell cycle regulators independently of p53.

AB - Nucleostemin (NS), a member of a family of nucleolar GTP-binding proteins, is highly expressed in proliferating cells such as stem and cancer cells and is involved in the control of cell cycle progression. Both depletion and overexpression of NS result in stabilization of the tumor suppressor p53 protein in vitro. Although it has been previously suggested that NS has p53-independent functions, these to date remain unknown. Here, we report two zebrafish mutants recovered from forward and reverse genetic screens that carry loss of function mutations in two members of this nucleolar protein family, Guanine nucleotide binding-protein-like 2 (Gnl2) and Gnl3/NS. We demonstrate that these proteins are required for correct timing of cell cycle exit and subsequent neural differentiation in the brain and retina. Concomitantly, we observe aberrant expression of the cell cycle regulators cyclinD1 and p57kip2. Our models demonstrate that the loss of Gnl2 or NS induces p53 stabilization and p53-mediated apoptosis. However, the retinal differentiation defects are independent of p53 activation. Furthermore, this work demonstrates that Gnl2 and NS have both non-cell autonomously and cell-autonomous function in correct timing of cell cycle exit and neural differentiation. Finally, the data suggest that Gnl2 and NS affect cell cycle exit of neural progenitors by regulating the expression of cell cycle regulators independently of p53.

KW - Animals

KW - Blotting, Western

KW - Bromodeoxyuridine

KW - Cell Cycle

KW - Cyclin D1

KW - Cyclin-Dependent Kinase Inhibitor p57

KW - GTP-Binding Proteins

KW - Gene Expression Regulation

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Microarray Analysis

KW - Microscopy, Fluorescence

KW - Mutation

KW - Neurogenesis

KW - Nuclear Proteins

KW - Oligonucleotides

KW - Plasmids

KW - Retina

KW - Zebrafish

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.ydbio.2011.04.028

DO - 10.1016/j.ydbio.2011.04.028

M3 - Article

VL - 355

SP - 286

EP - 301

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -

ID: 53430599