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The novel gene asb11: a regulator of the size of the neural progenitor compartment
Diks, S. H., Bink, R. J., van de Water, S., Joore, J., van Rooijen, C., Verbeek, F. J., den Hertog, J., Peppelenbosch, M. P. & Zivkovic, D., 14-Aug-2006, In : Journal of Cell Biology. 174, 4, p. 581-592 12 p.Research output: Contribution to journal › Article › Academic › peer-review
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The novel gene asb11 : a regulator of the size of the neural progenitor compartment. / Diks, Sander H.; Bink, Robert J.; van de Water, Sandra; Joore, Jos; van Rooijen, Carina; Verbeek, Fons J.; den Hertog, Jeroen; Peppelenbosch, Maikel P.; Zivkovic, Danica.
In: Journal of Cell Biology, Vol. 174, No. 4, 14.08.2006, p. 581-592.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - The novel gene asb11
T2 - a regulator of the size of the neural progenitor compartment
AU - Diks, Sander H.
AU - Bink, Robert J.
AU - van de Water, Sandra
AU - Joore, Jos
AU - van Rooijen, Carina
AU - Verbeek, Fons J.
AU - den Hertog, Jeroen
AU - Peppelenbosch, Maikel P.
AU - Zivkovic, Danica
N1 - Article J
PY - 2006/8/14
Y1 - 2006/8/14
N2 - From a differential display designed to isolate genes that are down-regulated upon differentiation of the central nervous system in Danio rerio embryos, we isolated d-asb11 ( ankyrin repeat and suppressor of cytokine signaling box-containing protein 11). Knockdown of the d-Asb11 protein altered the expression of neural precursor genes sox2 and sox3 and resulted in an initial relative increase in proneural cell numbers. This was reflected by neurogenin1 expansion followed by premature neuronal differentiation, as demonstrated by HuC labeling and resulting in reduced size of the definitive neuronal compartment. Forced misexpression of d-asb11 was capable of ectopically inducing sox2 while it diminished or entirely abolished neurogenesis. Overexpression of d-Asb11 in both a pluripotent and a neural-committed progenitor cell line resulted in the stimulus-induced inhibition of terminal neuronal differentiation and enhanced proliferation. We conclude that d-Asb11 is a novel regulator of the neuronal progenitor compartment size by maintaining the neural precursors in the proliferating undifferentiated state possibly through the control of SoxB1 transcription factors.
AB - From a differential display designed to isolate genes that are down-regulated upon differentiation of the central nervous system in Danio rerio embryos, we isolated d-asb11 ( ankyrin repeat and suppressor of cytokine signaling box-containing protein 11). Knockdown of the d-Asb11 protein altered the expression of neural precursor genes sox2 and sox3 and resulted in an initial relative increase in proneural cell numbers. This was reflected by neurogenin1 expansion followed by premature neuronal differentiation, as demonstrated by HuC labeling and resulting in reduced size of the definitive neuronal compartment. Forced misexpression of d-asb11 was capable of ectopically inducing sox2 while it diminished or entirely abolished neurogenesis. Overexpression of d-Asb11 in both a pluripotent and a neural-committed progenitor cell line resulted in the stimulus-induced inhibition of terminal neuronal differentiation and enhanced proliferation. We conclude that d-Asb11 is a novel regulator of the neuronal progenitor compartment size by maintaining the neural precursors in the proliferating undifferentiated state possibly through the control of SoxB1 transcription factors.
KW - CELL NUCLEAR FACTOR
KW - SOCS-BOX
KW - ANKYRIN REPEAT
KW - RETINOIC ACID
KW - ZEBRAFISH EMBRYO
KW - NERVOUS-SYSTEM
KW - STEM-CELLS/
KW - SOX GENES
KW - DIFFERENTIATION
KW - EXPRESSION
U2 - 10.1083/jcb.200601081
DO - 10.1083/jcb.200601081
M3 - Article
VL - 174
SP - 581
EP - 592
JO - The Journal of Cell Biology
JF - The Journal of Cell Biology
SN - 0021-9525
IS - 4
ER -
ID: 1522477