The lower matrix protein pp65 is the principal viral antigen present in peripheral blood leukocytes during an active cytomegalovirus infectionGrefte, J. M. M., van der Gun, B. F. T., Schmolke, S., van der Giessen, M., van Son, W. J., Plachter, B., Jahn, G. & The, T. H., Nov-1992, In : Journal of general virology. 73, p. 2923-2932 10 p.
Research output: Contribution to journal › Article › Academic › peer-review
During an active infection with human cytomegalovirus (HCMV), viral antigen is consistently present in peripheral blood leukocytes. Two monoclonal antibodies (MAbs), CMV-C10 and CMV-C11, are commonly used in the HCMV antigenaemia assay to detect these cells in the peripheral blood of patients suspected of having an active HCMV infection. We demonstrate that the viral antigen detected by these MAbs is the viral structural protein pp65 and not an immediate early antigen as previously reported. Furthermore, significantly fewer leukocytes were found to be positive with MAbs specific for immediate early antigens.
|Number of pages||10|
|Journal||Journal of general virology|
|Publication status||Published - Nov-1992|
- MONOCLONAL-ANTIBODY, INSITU HYBRIDIZATION, VIRUS INFECTIONS, KINASE ACTIVITY, GENE-PRODUCTS, EXPRESSION, TRANSPLANTATION, LOCALIZATION, VIREMIA, PP65