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The lower matrix protein pp65 is the principal viral antigen present in peripheral blood leukocytes during an active cytomegalovirus infection

Grefte, J. M. M., van der Gun, B. F. T., Schmolke, S., van der Giessen, M., van Son, W. J., Plachter, B., Jahn, G. & The, T. H., Nov-1992, In : Journal of general virology. 73, p. 2923-2932 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • J. M. M. Grefte
  • B. F. T. van der Gun
  • S. Schmolke
  • M. van der Giessen
  • W. J. van Son
  • B Plachter
  • G Jahn
  • T. Hauw The

During an active infection with human cytomegalovirus (HCMV), viral antigen is consistently present in peripheral blood leukocytes. Two monoclonal antibodies (MAbs), CMV-C10 and CMV-C11, are commonly used in the HCMV antigenaemia assay to detect these cells in the peripheral blood of patients suspected of having an active HCMV infection. We demonstrate that the viral antigen detected by these MAbs is the viral structural protein pp65 and not an immediate early antigen as previously reported. Furthermore, significantly fewer leukocytes were found to be positive with MAbs specific for immediate early antigens.

Original languageEnglish
Pages (from-to)2923-2932
Number of pages10
JournalJournal of general virology
Volume73
Publication statusPublished - Nov-1992

    Keywords

  • MONOCLONAL-ANTIBODY, INSITU HYBRIDIZATION, VIRUS INFECTIONS, KINASE ACTIVITY, GENE-PRODUCTS, EXPRESSION, TRANSPLANTATION, LOCALIZATION, VIREMIA, PP65

ID: 6330019