Publication

The lateral gate of SecYEG opens during protein translocation.

du Plessis, D. J. F., Berrelkamp, G., Nouwen, N. P. & Driessen, A. J. M., 5-Jun-2009, In : The Journal of Biological Chemistry. 284, 23, p. 15805-15814 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

The SecYEG translocon of Escherichia coli mediates the translocation of preproteins across the cytoplasmic membrane. Here, we have examined the role of the proposed lateral gate of the translocon in translocation. A dual cysteine cross-linking approach allowed the introduction of cross-linker arms of various lengths between adjoining aminoacyl positions of transmembrane segments 2b and 7 of the lateral gate. Oxidation and short spacer linkers that fix the gate in the closed state abolished preprotein translocation, whereas long spacer linkers support translocation. The cross-linking data further suggests that SecYEG lateral gate opening and activation of the SecA ATPase are coupled processes. It is concluded that lateral gate opening is a critical step during SecA-dependent protein translocation.

Original languageEnglish
Pages (from-to)15805-15814
Number of pages10
JournalThe Journal of Biological Chemistry
Volume284
Issue number23
Publication statusPublished - 5-Jun-2009

    Keywords

  • BACTERIAL CYTOPLASMIC MEMBRANE, ESCHERICHIA-COLI, CONDUCTING CHANNEL, PREPROTEIN TRANSLOCATION, CROSS-LINKING, CATALYTIC CYCLE, SECA PROTEIN, ATPASE, COMPLEX, OMPT

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