Publication

The impact of lemon pectin characteristics on TLR activation and T84 intestinal epithelial cell barrier function

Vogt, L. M., Sahasrabudhe, N. M., Ramasamy, U., Meyer, D., Pullens, G., Faas, M. M., Venema, K., Schols, H. A. & de Vos, P., Apr-2016, In : Journal of Functional Foods. 22, p. 398-407 10 p.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • The impact of lemon pectin characteristics on TLR activation

    Final publisher's version, 1 MB, PDF document

    Request copy

DOI

Sufficient dietary fibre intake reduces the risk of several diseases, but the mechanisms linking fibre structure and health effects remain unclear. To analyse the influence of the amount of methyl groups esterified to the backbone, lemon pectins of different degrees of methyl esterification (30, 56, and 74DM) were studied for immune receptor activating potential and epithelial barrier protection. In reporter assays, the pectins demonstrated TLR/MyD88 dependent activation of NF-kappa B/AP-1, which increased with increasing pectin DM. To analyse the importance of backbone structure, the pectins were enzymatically digested into oligomers, which abrogated TLR activating potential. The 30 and 74DM pectins induced strong protection of the epithelial barrier measured by T84 transepithelial electrical resistance (TEER). These results indicate that activation of immune cells by lemon pectins is TLR dependent, and the intact polymer backbone is indispensable for activation. In addition, DM is a determining factor in activating potential and epithelial barrier protective effects. (C) 2016 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)398-407
Number of pages10
JournalJournal of Functional Foods
Volume22
Publication statusPublished - Apr-2016

    Keywords

  • Pectin, Chain length, Methyl groups, TLR, NF-kappa B, Epithelial barrier, CITRUS PECTIN, DIETARY FIBER, RECEPTOR 2, ENDOPOLYGALACTURONASE, OLIGOSACCHARIDES, PURIFICATION, COMPLEX, CANCER, ENZYME

View graph of relations

ID: 41127176