Publication

The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid

Rosenling, T., Stoop, M. P., Smolinska, A., Muilwijk, B., Coulier, L., Shi, S., Dane, A., Christin, C., Suits, F., Horvatovich, P. L., Wijmenga, S. S., Buydens, L. M. C., Vreeken, R., Hankemeier, T., van Gool, A. J., Luider, T. M. & Bischoff, R., Dec-2011, In : Clinical Chemistry. 57, 12, p. 1703-1711 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Rosenling, T., Stoop, M. P., Smolinska, A., Muilwijk, B., Coulier, L., Shi, S., Dane, A., Christin, C., Suits, F., Horvatovich, P. L., Wijmenga, S. S., Buydens, L. M. C., Vreeken, R., Hankemeier, T., van Gool, A. J., Luider, T. M., & Bischoff, R. (2011). The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid. Clinical Chemistry, 57(12), 1703-1711. https://doi.org/10.1373/clinchem.2011.167601

Author

Rosenling, Therese ; Stoop, Marcel P. ; Smolinska, Agnieszka ; Muilwijk, Bas ; Coulier, Leon ; Shi, Shanna ; Dane, Adrie ; Christin, Christin ; Suits, Frank ; Horvatovich, Peter L. ; Wijmenga, Sybren S. ; Buydens, Lutgarde M. C. ; Vreeken, Rob ; Hankemeier, Thomas ; van Gool, Alain J. ; Luider, Theo M. ; Bischoff, Rainer. / The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid. In: Clinical Chemistry. 2011 ; Vol. 57, No. 12. pp. 1703-1711.

Harvard

Rosenling, T, Stoop, MP, Smolinska, A, Muilwijk, B, Coulier, L, Shi, S, Dane, A, Christin, C, Suits, F, Horvatovich, PL, Wijmenga, SS, Buydens, LMC, Vreeken, R, Hankemeier, T, van Gool, AJ, Luider, TM & Bischoff, R 2011, 'The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid', Clinical Chemistry, vol. 57, no. 12, pp. 1703-1711. https://doi.org/10.1373/clinchem.2011.167601

Standard

The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid. / Rosenling, Therese; Stoop, Marcel P.; Smolinska, Agnieszka; Muilwijk, Bas; Coulier, Leon; Shi, Shanna; Dane, Adrie; Christin, Christin; Suits, Frank; Horvatovich, Peter L.; Wijmenga, Sybren S.; Buydens, Lutgarde M. C.; Vreeken, Rob; Hankemeier, Thomas; van Gool, Alain J.; Luider, Theo M.; Bischoff, Rainer.

In: Clinical Chemistry, Vol. 57, No. 12, 12.2011, p. 1703-1711.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Rosenling T, Stoop MP, Smolinska A, Muilwijk B, Coulier L, Shi S et al. The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid. Clinical Chemistry. 2011 Dec;57(12):1703-1711. https://doi.org/10.1373/clinchem.2011.167601


BibTeX

@article{d8d7c3e641a7418d8497744788b3947d,
title = "The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid",
abstract = "BACKGROUND: Because cerebrospinal fluid (CSF) is in close contact with diseased areas in neurological disorders, it is an important source of material in the search for molecular biomarkers. However, sample handling for CSF collected from patients in a clinical setting might not always be adequate for use in proteomics and metabolomics studies.METHODS: We left CSF for 0, 30, and 120 min at room temperature immediately after sample collection and centrifugation/removal of cells. At 2 laboratories CSF proteomes were subjected to tryptic digestion and analyzed by use of nano-liquid chromatography (LC) Orbitrap mass spectrometry (MS) and chipLC quadrupole TOF-MS. Metabolome analysis was performed at 3 laboratories by NMR, GC-MS, and LC-MS. Targeted analyses of cystatin C and albumin were performed by LC-tandem MS in the selected reaction monitoring mode.RESULTS: We did not find significant changes in the measured proteome and metabolome of CSF stored at room temperature after centrifugation, except for 2 peptides and 1 metabolite, 2,3,4-trihydroxybutanoic (threonic) acid, of 5780 identified peptides and 93 identified metabolites. A sensitive protein stability marker, cystatin C, was not affected.CONCLUSIONS: The measured proteome and metabolome of centrifuged human CSF is stable at room temperature for up to 2 hours. We cannot exclude, however, that changes undetectable with our current methodology, such as denaturation or proteolysis, might occur because of sample handling conditions. The stability we observed gives laboratory personnel at the collection site sufficient time to aliquot samples before freezing and storage at -80 degrees C. (C) 2011 American Association for Clinical Chemistry",
keywords = "MULTIPLE-SCLEROSIS, MASS-SPECTROMETRY, LIQUID-CHROMATOGRAPHY, BIOMARKER DISCOVERY, NMR-SPECTROSCOPY, ASCORBIC-ACID, BLOOD-SERUM, CYSTATIN-C, CSF, STABILITY",
author = "Therese Rosenling and Stoop, {Marcel P.} and Agnieszka Smolinska and Bas Muilwijk and Leon Coulier and Shanna Shi and Adrie Dane and Christin Christin and Frank Suits and Horvatovich, {Peter L.} and Wijmenga, {Sybren S.} and Buydens, {Lutgarde M. C.} and Rob Vreeken and Thomas Hankemeier and {van Gool}, {Alain J.} and Luider, {Theo M.} and Rainer Bischoff",
year = "2011",
month = dec,
doi = "10.1373/clinchem.2011.167601",
language = "English",
volume = "57",
pages = "1703--1711",
journal = "Clinical Chemistry",
issn = "0009-9147",
publisher = "AMER ASSOC CLINICAL CHEMISTRY",
number = "12",

}

RIS

TY - JOUR

T1 - The Impact of Delayed Storage on the Measured Proteome and Metabolome of Human Cerebrospinal Fluid

AU - Rosenling, Therese

AU - Stoop, Marcel P.

AU - Smolinska, Agnieszka

AU - Muilwijk, Bas

AU - Coulier, Leon

AU - Shi, Shanna

AU - Dane, Adrie

AU - Christin, Christin

AU - Suits, Frank

AU - Horvatovich, Peter L.

AU - Wijmenga, Sybren S.

AU - Buydens, Lutgarde M. C.

AU - Vreeken, Rob

AU - Hankemeier, Thomas

AU - van Gool, Alain J.

AU - Luider, Theo M.

AU - Bischoff, Rainer

PY - 2011/12

Y1 - 2011/12

N2 - BACKGROUND: Because cerebrospinal fluid (CSF) is in close contact with diseased areas in neurological disorders, it is an important source of material in the search for molecular biomarkers. However, sample handling for CSF collected from patients in a clinical setting might not always be adequate for use in proteomics and metabolomics studies.METHODS: We left CSF for 0, 30, and 120 min at room temperature immediately after sample collection and centrifugation/removal of cells. At 2 laboratories CSF proteomes were subjected to tryptic digestion and analyzed by use of nano-liquid chromatography (LC) Orbitrap mass spectrometry (MS) and chipLC quadrupole TOF-MS. Metabolome analysis was performed at 3 laboratories by NMR, GC-MS, and LC-MS. Targeted analyses of cystatin C and albumin were performed by LC-tandem MS in the selected reaction monitoring mode.RESULTS: We did not find significant changes in the measured proteome and metabolome of CSF stored at room temperature after centrifugation, except for 2 peptides and 1 metabolite, 2,3,4-trihydroxybutanoic (threonic) acid, of 5780 identified peptides and 93 identified metabolites. A sensitive protein stability marker, cystatin C, was not affected.CONCLUSIONS: The measured proteome and metabolome of centrifuged human CSF is stable at room temperature for up to 2 hours. We cannot exclude, however, that changes undetectable with our current methodology, such as denaturation or proteolysis, might occur because of sample handling conditions. The stability we observed gives laboratory personnel at the collection site sufficient time to aliquot samples before freezing and storage at -80 degrees C. (C) 2011 American Association for Clinical Chemistry

AB - BACKGROUND: Because cerebrospinal fluid (CSF) is in close contact with diseased areas in neurological disorders, it is an important source of material in the search for molecular biomarkers. However, sample handling for CSF collected from patients in a clinical setting might not always be adequate for use in proteomics and metabolomics studies.METHODS: We left CSF for 0, 30, and 120 min at room temperature immediately after sample collection and centrifugation/removal of cells. At 2 laboratories CSF proteomes were subjected to tryptic digestion and analyzed by use of nano-liquid chromatography (LC) Orbitrap mass spectrometry (MS) and chipLC quadrupole TOF-MS. Metabolome analysis was performed at 3 laboratories by NMR, GC-MS, and LC-MS. Targeted analyses of cystatin C and albumin were performed by LC-tandem MS in the selected reaction monitoring mode.RESULTS: We did not find significant changes in the measured proteome and metabolome of CSF stored at room temperature after centrifugation, except for 2 peptides and 1 metabolite, 2,3,4-trihydroxybutanoic (threonic) acid, of 5780 identified peptides and 93 identified metabolites. A sensitive protein stability marker, cystatin C, was not affected.CONCLUSIONS: The measured proteome and metabolome of centrifuged human CSF is stable at room temperature for up to 2 hours. We cannot exclude, however, that changes undetectable with our current methodology, such as denaturation or proteolysis, might occur because of sample handling conditions. The stability we observed gives laboratory personnel at the collection site sufficient time to aliquot samples before freezing and storage at -80 degrees C. (C) 2011 American Association for Clinical Chemistry

KW - MULTIPLE-SCLEROSIS

KW - MASS-SPECTROMETRY

KW - LIQUID-CHROMATOGRAPHY

KW - BIOMARKER DISCOVERY

KW - NMR-SPECTROSCOPY

KW - ASCORBIC-ACID

KW - BLOOD-SERUM

KW - CYSTATIN-C

KW - CSF

KW - STABILITY

U2 - 10.1373/clinchem.2011.167601

DO - 10.1373/clinchem.2011.167601

M3 - Article

VL - 57

SP - 1703

EP - 1711

JO - Clinical Chemistry

JF - Clinical Chemistry

SN - 0009-9147

IS - 12

ER -

ID: 5462917