The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodiesvan Leeuwen, M., Damoiseaux, J., Duijvestijn, A., Heeringa, P., Gijbels, M., de Winther, M. & Tervaert, J. W. C., 2009, CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. Shoenfeld, Y. & Gershwin, ME. (eds.). MALDEN: Blackwell Publishing, p. 274-279 6 p. (Annals of the New York Academy of Sciences; vol. 1173).
Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review
Introduction: It is hypothesized that IgM antibodies to oxidized LDL are anti-atherogenic. Myeloperoxidase from plaque-infiltrating neutrophils catalyzes the production of hypochlorite (HOCl), which oxidizes LDL. Here we study the IgM response to HOCl-modified LDL in comparison to titers of T15 clonotypic natural antibodies. Methods: Plasma of LDLR-/- mice fed a normal chow or high-fat diet was obtained after 6 and 16 weeks. The IgM responses to HOCl-modified LDL and T15 clonotypic natural IgM antibodies were measured by ELISA. Results: The IgM levels in response to HOCl-modified LDL increased dramatically in the atherosclerotic group after introduction of the high-fat diet, but not in mice on normal chow. The natural IgM T15 clonotypic antibody titers revealed a more moderate increase during atherogenesis. Conclusion: Our results show that during atherogenesis there is a strong induction of IgM antibodies to HOCl-modified LDL particles. Whether these induced IgM antibodies are pro- or anti-atherogenic remains to be established.
|Title of host publication||CONTEMPORARY CHALLENGES IN AUTOIMMUNITY|
|Editors||Y Shoenfeld, ME Gershwin|
|Place of Publication||MALDEN|
|Number of pages||6|
|Publication status||Published - 2009|
|Event||6th International Congress on Autoimmunity - , Portugal|
Duration: 10-Sep-2008 → 14-Sep-2008
|Name||Annals of the New York Academy of Sciences|
|Other||6th International Congress on Autoimmunity|
|Period||10/09/2008 → 14/09/2008|
6th International Congress on Autoimmunity
10/09/2008 → 14/09/2008Portugal
- atherosclerosis, mouse, IgM antibodies, HOCl-modified LDL, LOW-DENSITY-LIPOPROTEIN, CORONARY-ARTERY-DISEASE, OXIDIZED LDL, IMMUNE-COMPLEXES, MYELOPEROXIDASE, EPITOPES, OXIDATION, LESIONS, MACROPHAGES, CELLS