Publication

The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies

van Leeuwen, M., Damoiseaux, J., Duijvestijn, A., Heeringa, P., Gijbels, M., de Winther, M. & Tervaert, J. W. C., 2009, CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. Shoenfeld, Y. & Gershwin, ME. (eds.). MALDEN: Blackwell Publishing, p. 274-279 6 p. (Annals of the New York Academy of Sciences; vol. 1173).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Introduction: It is hypothesized that IgM antibodies to oxidized LDL are anti-atherogenic. Myeloperoxidase from plaque-infiltrating neutrophils catalyzes the production of hypochlorite (HOCl), which oxidizes LDL. Here we study the IgM response to HOCl-modified LDL in comparison to titers of T15 clonotypic natural antibodies. Methods: Plasma of LDLR-/- mice fed a normal chow or high-fat diet was obtained after 6 and 16 weeks. The IgM responses to HOCl-modified LDL and T15 clonotypic natural IgM antibodies were measured by ELISA. Results: The IgM levels in response to HOCl-modified LDL increased dramatically in the atherosclerotic group after introduction of the high-fat diet, but not in mice on normal chow. The natural IgM T15 clonotypic antibody titers revealed a more moderate increase during atherogenesis. Conclusion: Our results show that during atherogenesis there is a strong induction of IgM antibodies to HOCl-modified LDL particles. Whether these induced IgM antibodies are pro- or anti-atherogenic remains to be established.

Original languageEnglish
Title of host publicationCONTEMPORARY CHALLENGES IN AUTOIMMUNITY
EditorsY Shoenfeld, ME Gershwin
Place of PublicationMALDEN
PublisherBlackwell Publishing
Pages274-279
Number of pages6
ISBN (Print)978-1-57331-762-7
Publication statusPublished - 2009
Event6th International Congress on Autoimmunity - , Portugal
Duration: 10-Sep-200814-Sep-2008

Publication series

NameAnnals of the New York Academy of Sciences
PublisherWILEY-BLACKWELL
Volume1173
ISSN (Print)0077-8923

Other

Other6th International Congress on Autoimmunity
CountryPortugal
Period10/09/200814/09/2008

Event

6th International Congress on Autoimmunity

10/09/200814/09/2008

Portugal

Event: Other

    Keywords

  • atherosclerosis, mouse, IgM antibodies, HOCl-modified LDL, LOW-DENSITY-LIPOPROTEIN, CORONARY-ARTERY-DISEASE, OXIDIZED LDL, IMMUNE-COMPLEXES, MYELOPEROXIDASE, EPITOPES, OXIDATION, LESIONS, MACROPHAGES, CELLS

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ID: 4969533