Publication

The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano

Demircan, T. & Berezikov, E., Aug-2013, In : Stem cells and development. 22, 15, p. 2174-2185 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Demircan, T., & Berezikov, E. (2013). The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano. Stem cells and development, 22(15), 2174-2185. https://doi.org/10.1089/scd.2013.0006

Author

Demircan, Turan ; Berezikov, Eugene. / The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano. In: Stem cells and development. 2013 ; Vol. 22, No. 15. pp. 2174-2185.

Harvard

Demircan, T & Berezikov, E 2013, 'The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano', Stem cells and development, vol. 22, no. 15, pp. 2174-2185. https://doi.org/10.1089/scd.2013.0006

Standard

The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano. / Demircan, Turan; Berezikov, Eugene.

In: Stem cells and development, Vol. 22, No. 15, 08.2013, p. 2174-2185.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Demircan T, Berezikov E. The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano. Stem cells and development. 2013 Aug;22(15):2174-2185. https://doi.org/10.1089/scd.2013.0006


BibTeX

@article{83eb65d5f365434ba257e8ef5304a1fe,
title = "The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano",
abstract = "The Hippo pathway orchestrates activity of stem cells during development and tissue regeneration and is crucial for controlling organ size. However, roles of the Hippo pathway in highly regenerative organisms, such as flatworms, are unknown. Here we show that knockdown of the Hippo pathway core genes in the flatworm Macrostomum lignano affects tissue homeostasis and causes formation of outgrowths through hyperproliferation of stem cells (neoblasts), and leads to disruption of allometric scaling during regeneration and increased size of regenerated parts. We further show that Yap, the downstream effector of the Hippo pathway, is a potential neoblast marker gene, as it is expressed in dividing cells in M. lignano and is essential for neoblast self-renewal. The phenotypes we observe in M. lignano upon knockdown of the Hippo pathway core genes and Yap are consistent with the known functions of the pathway in other model organisms and demonstrate that the Hippo pathway is functionally conserved between flatworms and mammals. This work establishes M. lignano as a productive model for investigation of the Hippo pathway.",
keywords = "ORGAN SIZE CONTROL, TUMOR-SUPPRESSOR, SELF-RENEWAL, PROMOTES APOPTOSIS, PLANARIAN REGENERATION, PROLIFERATION ARREST, SIGNALING PATHWAY, PROTEIN-KINASE, YAP PATHWAY, CYCLE EXIT",
author = "Turan Demircan and Eugene Berezikov",
year = "2013",
month = aug,
doi = "10.1089/scd.2013.0006",
language = "English",
volume = "22",
pages = "2174--2185",
journal = "Stem cells and development",
issn = "1547-3287",
publisher = "MARY ANN LIEBERT, INC",
number = "15",

}

RIS

TY - JOUR

T1 - The Hippo Pathway Regulates Stem Cells During Homeostasis and Regeneration of the Flatworm Macrostomum Lignano

AU - Demircan, Turan

AU - Berezikov, Eugene

PY - 2013/8

Y1 - 2013/8

N2 - The Hippo pathway orchestrates activity of stem cells during development and tissue regeneration and is crucial for controlling organ size. However, roles of the Hippo pathway in highly regenerative organisms, such as flatworms, are unknown. Here we show that knockdown of the Hippo pathway core genes in the flatworm Macrostomum lignano affects tissue homeostasis and causes formation of outgrowths through hyperproliferation of stem cells (neoblasts), and leads to disruption of allometric scaling during regeneration and increased size of regenerated parts. We further show that Yap, the downstream effector of the Hippo pathway, is a potential neoblast marker gene, as it is expressed in dividing cells in M. lignano and is essential for neoblast self-renewal. The phenotypes we observe in M. lignano upon knockdown of the Hippo pathway core genes and Yap are consistent with the known functions of the pathway in other model organisms and demonstrate that the Hippo pathway is functionally conserved between flatworms and mammals. This work establishes M. lignano as a productive model for investigation of the Hippo pathway.

AB - The Hippo pathway orchestrates activity of stem cells during development and tissue regeneration and is crucial for controlling organ size. However, roles of the Hippo pathway in highly regenerative organisms, such as flatworms, are unknown. Here we show that knockdown of the Hippo pathway core genes in the flatworm Macrostomum lignano affects tissue homeostasis and causes formation of outgrowths through hyperproliferation of stem cells (neoblasts), and leads to disruption of allometric scaling during regeneration and increased size of regenerated parts. We further show that Yap, the downstream effector of the Hippo pathway, is a potential neoblast marker gene, as it is expressed in dividing cells in M. lignano and is essential for neoblast self-renewal. The phenotypes we observe in M. lignano upon knockdown of the Hippo pathway core genes and Yap are consistent with the known functions of the pathway in other model organisms and demonstrate that the Hippo pathway is functionally conserved between flatworms and mammals. This work establishes M. lignano as a productive model for investigation of the Hippo pathway.

KW - ORGAN SIZE CONTROL

KW - TUMOR-SUPPRESSOR

KW - SELF-RENEWAL

KW - PROMOTES APOPTOSIS

KW - PLANARIAN REGENERATION

KW - PROLIFERATION ARREST

KW - SIGNALING PATHWAY

KW - PROTEIN-KINASE

KW - YAP PATHWAY

KW - CYCLE EXIT

U2 - 10.1089/scd.2013.0006

DO - 10.1089/scd.2013.0006

M3 - Article

VL - 22

SP - 2174

EP - 2185

JO - Stem cells and development

JF - Stem cells and development

SN - 1547-3287

IS - 15

ER -

ID: 5915989