The Hansenula polymorpha ATG25 gene encodes a novel coiled-coil protein that is required for macropexophagyMonastyrska, I., Kiel, J. A. K. W., Krikken, A. M., Komduur, J. A., Veenhuis, M. & van der Klei, I. J., 2005, In : Autophagy. 1, 2, p. 92 - 100 9 p.
Research output: Contribution to journal › Article › Academic › peer-review
We have isolated the Hansenula polymorpha ATG25 gene, which is required for glucose-induced selective peroxisome degradation by macropexophagy. ATG25 represents a novel gene that encodes a 45 kDa coiled-coil protein. We show that this protein colocalizes with Atg11 on a small structure, which most likely represents the pre-autophagosomal structure (PAS). In cells of a constructed ATG25 deletion strain (atg25) peroxisomes are constitutively degraded by nonselective microautophagy, a process that in WT H. polymorpha is only observed at nitrogen limitation conditions. This suggests that nonselective microautophagy is deregulated in H. polymorpha atg25 cells.
|Pages (from-to)||92 - 100|
|Number of pages||9|
|Publication status||Published - 2005|
- yeast, microautophagy, pexophagy, ATG 11, ATG25, pre-autophagosomal structure, YEAST HANSENULA-POLYMORPHA, VACUOLE TARGETING PATHWAY, SELECTIVE DEGRADATION, PEROXISOME DEGRADATION, PICHIA-PASTORIS, BIOGENESIS, AUTOPHAGY, PROLIFERATION, CYTOPLASM, ENZYME