Publication

The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts

Donker, R. B., Mouillet, J-F., Nelson, D. M. & Sadovsky, Y., Apr-2007, In : Molecular human reproduction. 13, 4, p. 273-279 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Donker, R. B., Mouillet, J-F., Nelson, D. M., & Sadovsky, Y. (2007). The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. Molecular human reproduction, 13(4), 273-279. https://doi.org/10.1093/molehr/gam006

Author

Donker, Rogier B. ; Mouillet, Jean-Francois ; Nelson, D. Michael ; Sadovsky, Yoel. / The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. In: Molecular human reproduction. 2007 ; Vol. 13, No. 4. pp. 273-279.

Harvard

Donker, RB, Mouillet, J-F, Nelson, DM & Sadovsky, Y 2007, 'The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts', Molecular human reproduction, vol. 13, no. 4, pp. 273-279. https://doi.org/10.1093/molehr/gam006

Standard

The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. / Donker, Rogier B.; Mouillet, Jean-Francois; Nelson, D. Michael; Sadovsky, Yoel.

In: Molecular human reproduction, Vol. 13, No. 4, 04.2007, p. 273-279.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Donker RB, Mouillet J-F, Nelson DM, Sadovsky Y. The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts. Molecular human reproduction. 2007 Apr;13(4):273-279. https://doi.org/10.1093/molehr/gam006


BibTeX

@article{01f918f5788242799700c000554be8b6,
title = "The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts",
abstract = "Endogenous microRNAs (miRNAs) post-transcriptionally regulate mRNA and protein expression during tissue development and function. Whereas adaptation to environmental insults are tightly regulated in human tissues, the role of miRNAs and miRNA biogenesis proteins in this context is inadequately explored. We sought to analyse the expression of the key RNAi enzyme Argonaute2 (Ago2) and other miRNA biogenesis proteins in human trophoblasts during differentiation and in hypoxic environment. Using an in vitro analysis of primary term human trophoblasts, we identified the expression of the core miRNA biogenesis proteins in human villous trophoblasts, with expression levels unaffected by cellular differentiation. We found that the miRNA biosynthetic pathway was functional and produced miRNAs, with miR-93 up-regulated and miR-424 down-regulated in hypoxic environment. In contrast, hypoxia did not alter the expression of key miRNA machinery proteins. The pivotal miRNA processing enzyme Ago2, along with its interacting protein DP103, were expressed in normal placentas as well as in placentas from pregnancies complicated by placental hypoperfusion that resulted in fetal growth restriction. Ago2 and DP103 co-immunoprecipitated, and did not limit trophoblast response to hypoxic stress. We concluded that the core miRNA machinery proteins are expressed and functional in human trophoblasts. The influence of hypoxia on the expression of a subset of placental miRNA species is unlikely to reflect altered expression of key miRNA biogenesis proteins.",
keywords = "Ago2, hypoxia, microRNA biogenesis, placenta, trophoblast, TERM HUMAN TROPHOBLASTS, MESSENGER-RNAS, HUMAN-CELLS, GENE-EXPRESSION, HUMAN PLACENTA, DIFFERENTIATION, COMPLEX, IDENTIFICATION, REPRESSION, RIBONUCLEOPROTEINS",
author = "Donker, {Rogier B.} and Jean-Francois Mouillet and Nelson, {D. Michael} and Yoel Sadovsky",
year = "2007",
month = "4",
doi = "10.1093/molehr/gam006",
language = "English",
volume = "13",
pages = "273--279",
journal = "Molecular human reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "4",

}

RIS

TY - JOUR

T1 - The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts

AU - Donker, Rogier B.

AU - Mouillet, Jean-Francois

AU - Nelson, D. Michael

AU - Sadovsky, Yoel

PY - 2007/4

Y1 - 2007/4

N2 - Endogenous microRNAs (miRNAs) post-transcriptionally regulate mRNA and protein expression during tissue development and function. Whereas adaptation to environmental insults are tightly regulated in human tissues, the role of miRNAs and miRNA biogenesis proteins in this context is inadequately explored. We sought to analyse the expression of the key RNAi enzyme Argonaute2 (Ago2) and other miRNA biogenesis proteins in human trophoblasts during differentiation and in hypoxic environment. Using an in vitro analysis of primary term human trophoblasts, we identified the expression of the core miRNA biogenesis proteins in human villous trophoblasts, with expression levels unaffected by cellular differentiation. We found that the miRNA biosynthetic pathway was functional and produced miRNAs, with miR-93 up-regulated and miR-424 down-regulated in hypoxic environment. In contrast, hypoxia did not alter the expression of key miRNA machinery proteins. The pivotal miRNA processing enzyme Ago2, along with its interacting protein DP103, were expressed in normal placentas as well as in placentas from pregnancies complicated by placental hypoperfusion that resulted in fetal growth restriction. Ago2 and DP103 co-immunoprecipitated, and did not limit trophoblast response to hypoxic stress. We concluded that the core miRNA machinery proteins are expressed and functional in human trophoblasts. The influence of hypoxia on the expression of a subset of placental miRNA species is unlikely to reflect altered expression of key miRNA biogenesis proteins.

AB - Endogenous microRNAs (miRNAs) post-transcriptionally regulate mRNA and protein expression during tissue development and function. Whereas adaptation to environmental insults are tightly regulated in human tissues, the role of miRNAs and miRNA biogenesis proteins in this context is inadequately explored. We sought to analyse the expression of the key RNAi enzyme Argonaute2 (Ago2) and other miRNA biogenesis proteins in human trophoblasts during differentiation and in hypoxic environment. Using an in vitro analysis of primary term human trophoblasts, we identified the expression of the core miRNA biogenesis proteins in human villous trophoblasts, with expression levels unaffected by cellular differentiation. We found that the miRNA biosynthetic pathway was functional and produced miRNAs, with miR-93 up-regulated and miR-424 down-regulated in hypoxic environment. In contrast, hypoxia did not alter the expression of key miRNA machinery proteins. The pivotal miRNA processing enzyme Ago2, along with its interacting protein DP103, were expressed in normal placentas as well as in placentas from pregnancies complicated by placental hypoperfusion that resulted in fetal growth restriction. Ago2 and DP103 co-immunoprecipitated, and did not limit trophoblast response to hypoxic stress. We concluded that the core miRNA machinery proteins are expressed and functional in human trophoblasts. The influence of hypoxia on the expression of a subset of placental miRNA species is unlikely to reflect altered expression of key miRNA biogenesis proteins.

KW - Ago2

KW - hypoxia

KW - microRNA biogenesis

KW - placenta

KW - trophoblast

KW - TERM HUMAN TROPHOBLASTS

KW - MESSENGER-RNAS

KW - HUMAN-CELLS

KW - GENE-EXPRESSION

KW - HUMAN PLACENTA

KW - DIFFERENTIATION

KW - COMPLEX

KW - IDENTIFICATION

KW - REPRESSION

KW - RIBONUCLEOPROTEINS

U2 - 10.1093/molehr/gam006

DO - 10.1093/molehr/gam006

M3 - Article

VL - 13

SP - 273

EP - 279

JO - Molecular human reproduction

JF - Molecular human reproduction

SN - 1360-9947

IS - 4

ER -

ID: 4548009