Publication

The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices

Ruigrok, M., Tomar, J., Frijlink, H. W., Melgert, B., Hinrichs, W. & Olinga, P., 7-Nov-2019, In : Scientific Reports. 9, 1, 13 p., 16239.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Ruigrok, M., Tomar, J., Frijlink, H. W., Melgert, B., Hinrichs, W., & Olinga, P. (2019). The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices. Scientific Reports, 9(1), [16239]. https://doi.org/10.1038/s41598-019-52813-2

Author

Ruigrok, Mitchel ; Tomar, Jasmine ; Frijlink, H.W. ; Melgert, Barbro ; Hinrichs, Wouter ; Olinga, Peter. / The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices. In: Scientific Reports. 2019 ; Vol. 9, No. 1.

Harvard

Ruigrok, M, Tomar, J, Frijlink, HW, Melgert, B, Hinrichs, W & Olinga, P 2019, 'The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices', Scientific Reports, vol. 9, no. 1, 16239. https://doi.org/10.1038/s41598-019-52813-2

Standard

The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices. / Ruigrok, Mitchel; Tomar, Jasmine; Frijlink, H.W.; Melgert, Barbro; Hinrichs, Wouter; Olinga, Peter.

In: Scientific Reports, Vol. 9, No. 1, 16239, 07.11.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Ruigrok M, Tomar J, Frijlink HW, Melgert B, Hinrichs W, Olinga P. The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices. Scientific Reports. 2019 Nov 7;9(1). 16239. https://doi.org/10.1038/s41598-019-52813-2


BibTeX

@article{ecb6f1cbd1524e9784b8da6fef642600,
title = "The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices",
abstract = "Although animal models are often used in drug research, alternative experimental models are becoming more popular as they reduce animal use and suffering. Of particular interest are precision-cut lung slices, which refer to explants - with a reproducible thickness and diameter - that can be cultured ex vivo. Because lung slices (partially) reflect functional and structural features of whole tissue, they are often applied in the field of immunology, pharmacology, toxicology, and virology. Nevertheless, previous research failed to adequately address concerns with respect to the viability of lung slices. For instance, the effect of oxygen concentration on lung slice viability has never been thoroughly investigated. Therefore, the main goal of this study was to investigate the effect of oxygen concentration (20 vs. 80{\%} O-2) on the degree of cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in lung slices. According to the results, slices incubated at 20{\%} O-2 displayed less cell death, antioxidant transcription, and acute inflammation, as well as more cell proliferation, demonstrating that these slices were considerably more viable than slices cultured at 80{\%} O-2. These findings expand our knowledge on lung slices and their use as an alternative experimental model in drug research.",
keywords = "EX-VIVO, APOPTOSIS, MECHANISMS, NECROSIS, REPAIR, LIVER, MODEL",
author = "Mitchel Ruigrok and Jasmine Tomar and H.W. Frijlink and Barbro Melgert and Wouter Hinrichs and Peter Olinga",
year = "2019",
month = "11",
day = "7",
doi = "10.1038/s41598-019-52813-2",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - The effects of oxygen concentration on cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices

AU - Ruigrok, Mitchel

AU - Tomar, Jasmine

AU - Frijlink, H.W.

AU - Melgert, Barbro

AU - Hinrichs, Wouter

AU - Olinga, Peter

PY - 2019/11/7

Y1 - 2019/11/7

N2 - Although animal models are often used in drug research, alternative experimental models are becoming more popular as they reduce animal use and suffering. Of particular interest are precision-cut lung slices, which refer to explants - with a reproducible thickness and diameter - that can be cultured ex vivo. Because lung slices (partially) reflect functional and structural features of whole tissue, they are often applied in the field of immunology, pharmacology, toxicology, and virology. Nevertheless, previous research failed to adequately address concerns with respect to the viability of lung slices. For instance, the effect of oxygen concentration on lung slice viability has never been thoroughly investigated. Therefore, the main goal of this study was to investigate the effect of oxygen concentration (20 vs. 80% O-2) on the degree of cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in lung slices. According to the results, slices incubated at 20% O-2 displayed less cell death, antioxidant transcription, and acute inflammation, as well as more cell proliferation, demonstrating that these slices were considerably more viable than slices cultured at 80% O-2. These findings expand our knowledge on lung slices and their use as an alternative experimental model in drug research.

AB - Although animal models are often used in drug research, alternative experimental models are becoming more popular as they reduce animal use and suffering. Of particular interest are precision-cut lung slices, which refer to explants - with a reproducible thickness and diameter - that can be cultured ex vivo. Because lung slices (partially) reflect functional and structural features of whole tissue, they are often applied in the field of immunology, pharmacology, toxicology, and virology. Nevertheless, previous research failed to adequately address concerns with respect to the viability of lung slices. For instance, the effect of oxygen concentration on lung slice viability has never been thoroughly investigated. Therefore, the main goal of this study was to investigate the effect of oxygen concentration (20 vs. 80% O-2) on the degree of cell death, anti-oxidant transcription, acute inflammation, and cell proliferation in lung slices. According to the results, slices incubated at 20% O-2 displayed less cell death, antioxidant transcription, and acute inflammation, as well as more cell proliferation, demonstrating that these slices were considerably more viable than slices cultured at 80% O-2. These findings expand our knowledge on lung slices and their use as an alternative experimental model in drug research.

KW - EX-VIVO

KW - APOPTOSIS

KW - MECHANISMS

KW - NECROSIS

KW - REPAIR

KW - LIVER

KW - MODEL

U2 - 10.1038/s41598-019-52813-2

DO - 10.1038/s41598-019-52813-2

M3 - Article

C2 - 31700101

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 16239

ER -

ID: 101554439