The effect of Porphyromonas gingivalis lipopolysaccharide on pregnancy in the ratKunnen, A., van Pampus, M. G., Aarnoudse, J. G., van der Schans, C. P., Abbas, F. & Faas, M. M., Sep-2014, In : Oral diseases. 20, 6, p. 591-601 11 p.
Research output: Contribution to journal › Article › Academic › peer-review
OBJECTIVE: Periodontitis, mostly associated with Porphyromonas gingivalis, has frequently been related to adverse pregnancy outcomes. We therefore investigated whether lipopolysaccharides of P. gingivalis (Pg-LPS) induced pregnancy complications in the rat.
METHODS: Experiment 1: pregnant rats (day 14) received increasing Pg-LPS doses (0.0-50.0 μg kg(-1) bw; n = 2/3 p per dose). Maternal intra-aortic blood pressure, urinary albumin excretion, placental and foetal weight and foetal resorptions were documented. Experiment 2: 10.0 μg kg(-1) bw (which induced the highest blood pressure together with decreased foetal weight in experiment 1) or saline was infused in pregnant and non-pregnant rats (n = 7/9 p per group). Parameters of experiment 1 and numbers of peripheral leucocytes as well as signs of inflammation in the kidney and placenta were evaluated.
RESULTS: Pg-LPS infusion in pregnant rats increased maternal systolic blood pressure, reduced placental weight (dose dependently) and decreased foetal weight and induced foetal resorptions. It, however, did not induce proteinuria or a generalised inflammatory response. No effects of Pg-LPS were seen in non-pregnant rats.
CONCLUSION: Pg-LPS increased maternal blood pressure, induced placental and foetal growth restriction, and increased foetal resorptions, without inducing proteinuria and inflammation. Pg-LPS may therefore play a role in pregnancy complications induced by periodontitis.
|Number of pages||11|
|Publication status||Published - Sep-2014|
- periodontitis, pregnancy complications, rat, hypertension, LOW-DOSE ENDOTOXIN, FETAL-GROWTH RESTRICTION, EXPERIMENTAL PREECLAMPSIA, TROPHOBLAST RESPONSES, PERIODONTAL PATHOGENS, ESCHERICHIA-COLI, TNF-ALPHA, HYPERTENSION, INFLAMMATION, INFECTION