Publication

The DNAJB6 and DNAJB8 Protein Chaperones Prevent Intracellular Aggregation of Polyglutamine Peptides

Gillis, J., Schipper-Krom, S., Juenemann, K., Gruber, A., Coolen, S., van den Nieuwendijk, R., van Veen, H., Overkleeft, H., Goedhart, J., Kampinga, H. H. & Reits, E. A., 14-Jun-2013, In : The Journal of Biological Chemistry. 288, 24, p. 17225-17237 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Judith Gillis
  • Sabine Schipper-Krom
  • Katrin Juenemann
  • Anna Gruber
  • Silvia Coolen
  • Rian van den Nieuwendijk
  • Henk van Veen
  • Hermen Overkleeft
  • Joachim Goedhart
  • Harm H. Kampinga
  • Eric A. Reits

Fragments of proteins containing an expanded polyglutamine (polyQ) tract are thought to initiate aggregation and toxicity in at least nine neurodegenerative diseases, including Huntington's disease. Because proteasomes appear unable to digest the polyQ tract, which can initiate intracellular protein aggregation, preventing polyQ peptide aggregation by chaperones should greatly improve polyQ clearance and prevent aggregate formation. Here we expressed polyQ peptides in cells and show that their intracellular aggregation is prevented by DNAJB6 and DNAJB8, members of the DNAJ (Hsp40) chaperone family. In contrast, HSPA/Hsp70 and DNAJB1, also members of the DNAJ chaperone family, did not prevent peptide-initiated aggregation. Intriguingly, DNAJB6 and DNAJB8 also affected the soluble levels of polyQ peptides, indicating that DNAJB6 and DNAJB8 inhibit polyQ peptide aggregation directly. Together with recent data showing that purified DNAJB6 can suppress fibrillation of polyQ peptides far more efficiently than polyQ expanded protein fragments in vitro, we conclude that the mechanism of DNAJB6 and DNAJB8 is suppression of polyQ protein aggregation by directly binding the polyQ tract.

Original languageEnglish
Pages (from-to)17225-17237
Number of pages13
JournalThe Journal of Biological Chemistry
Volume288
Issue number24
Publication statusPublished - 14-Jun-2013

    Keywords

  • LIFETIME IMAGING MICROSCOPY, BULBAR MUSCULAR-ATROPHY, HEAT-SHOCK PROTEINS, EXPANDED POLYGLUTAMINE, MOLECULAR CHAPERONES, ANDROGEN RECEPTOR, PROTEOLYTIC CLEAVAGE, NUCLEAR INCLUSIONS, MUTANT HUNTINGTIN, MAMMALIAN-CELLS

ID: 5887992