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THE COMBI-EFFECT - REDUCED REJECTION OF THE HEART BY COMBINED TRANSPLANTATION WITH THE LUNG OR SPLEEN

WESTRA, AL., PETERSEN, AH., PROP, J. & WILDEVUUR, CRH., Dec-1991, In : Transplantation. 52, 6, p. 952-955 4 p.

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The term combi-effect was introduced to describe the phenomenon of a reduction in rejection of heart grafts after combined transplantation with the lung. In this study in rats we investigated whether the combi-effect was an immunological process and whether it could also be induced by combined transplantation of the heart with the spleen or with a lymphocyte-depleted spleen.

Heart and spleen grafts were transplanted into the abdomen; left lungs were transplanted into the thorax of recipient rats. To deplete spleens of their lymphocytes, prospective donor rats were irradiated. Cyclosporine was injected once, on day 2 after transplantation.

All heart allografts transplanted alone and treated with cyclosporine were rejected acutely (median survival time [MST] of 14.5 days). In contrast, after combined transplantation of a donor lung or spleen with the heart, almost all heart grafts survived indefinitely. Transplantation of a syngeneic lung or third-party spleen had little effect on heart graft survival (MST of 22.5 days and 26.5 days, respectively). Without cyclosporine treatment, combined transplantation with a donor lung or spleen hardly prolonged heart graft survival. Transplantation of a lymphocyte-depleted spleen with the heart induced a combi-effect in cyclosporine-treated rats that was somewhat weaker: only two of six hearts survived indefinitely.

We conclude that in the combi-effect an immunological mechanism reduces rejection of the heart. This mechanism is probably generated by the lymphoid tissue (bronchus-associated lymphoid tissue in lung and white pulp in spleen) in the combined transplant.

Original languageEnglish
Pages (from-to)952-955
Number of pages4
JournalTransplantation
Volume52
Issue number6
Publication statusPublished - Dec-1991

    Keywords

  • SUPPRESSOR CELLS, LYMPHOID-TISSUE, MONOCLONAL-ANTIBODIES, PROLONGED SURVIVAL, RAT, CYCLOSPORINE, ALLOGRAFT, GRAFTS, INDUCTION

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