Publication

The additional value of real-time PCR in the quantitative detection of periodontal pathogens

Boutaga, K., Van Winkelhoff, AJ., Vandenbroucke-Grauls, CMJE. & Savelkoul, PHM., Jun-2006, In : Journal of Clinical Periodontology. 33, 6, p. 427-433 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Boutaga, K., Van Winkelhoff, AJ., Vandenbroucke-Grauls, CMJE., & Savelkoul, PHM. (2006). The additional value of real-time PCR in the quantitative detection of periodontal pathogens. Journal of Clinical Periodontology, 33(6), 427-433. https://doi.org/10.1111/j.1600-051X.2006.00925.x

Author

Boutaga, K ; Van Winkelhoff, AJ ; Vandenbroucke-Grauls, CMJE ; Savelkoul, PHM. / The additional value of real-time PCR in the quantitative detection of periodontal pathogens. In: Journal of Clinical Periodontology. 2006 ; Vol. 33, No. 6. pp. 427-433.

Harvard

Boutaga, K, Van Winkelhoff, AJ, Vandenbroucke-Grauls, CMJE & Savelkoul, PHM 2006, 'The additional value of real-time PCR in the quantitative detection of periodontal pathogens', Journal of Clinical Periodontology, vol. 33, no. 6, pp. 427-433. https://doi.org/10.1111/j.1600-051X.2006.00925.x

Standard

The additional value of real-time PCR in the quantitative detection of periodontal pathogens. / Boutaga, K; Van Winkelhoff, AJ; Vandenbroucke-Grauls, CMJE; Savelkoul, PHM.

In: Journal of Clinical Periodontology, Vol. 33, No. 6, 06.2006, p. 427-433.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Boutaga K, Van Winkelhoff AJ, Vandenbroucke-Grauls CMJE, Savelkoul PHM. The additional value of real-time PCR in the quantitative detection of periodontal pathogens. Journal of Clinical Periodontology. 2006 Jun;33(6):427-433. https://doi.org/10.1111/j.1600-051X.2006.00925.x


BibTeX

@article{3ac749f40aaa4c7cb51dc2c869fe5d4e,
title = "The additional value of real-time PCR in the quantitative detection of periodontal pathogens",
abstract = "Background and Aim: For the analysis of subgingival plaque, anaerobic bacterial culture has been the gold standard for many years. Currently, molecular microbial techniques have become available to identify and quantify target organisms with high specificity and sensitivity. The technique of real-time polymerase chain reaction (RT-PCR) provides a new tool to detect oral pathogens both in oral and non-oral human infections. The aim of this study was to compare the RT-PCR and anaerobic culture for detection and quantification of six periodontal pathogens in periodontal health and disease.Material and Methods: Subgingival plaque samples from 259 adult patients with periodontitis and 111 healthy controls were analysed with quantitative anaerobic culture and quantitative RT-PCR for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Micromonas micros and Fusobacterium spp.Results: All species were more frequently isolated from patients than controls with both culture and RT-PCR. P. gingivalis, T. forsythia and M. micros appeared significant markers for disease with both techniques. P. intermedia was significantly associated with periodontitis by RT-PCR only (OR 9.7), whereas A. actinomycetemcomitans showed a significant relationship by culture only. The critical differences between culture and RT-PCR were culture-negative/PCR-positive samples which amounted to 7% for A. actinomycetemcomitans, 3% for P. gingivalis, 7% for T. forsythia, 20% for P. intermedia, 6% for M. micros, and 0.8% for Fusobacterium spp. in periodontitis patients and 12%, 3%, 2%, 35%, 14% and 0%, respectively, in the periodontally healthy group. Furthermore, periodontitis individuals had significantly higher amount of all of the test species in the subgingival plaque samples compared with healthy subjects.Conclusion: RT-PCR provides a new rapid diagnostic tool and opens the opportunity to detect small numbers of oral pathogens in clinical specimens, which are under the detection limit by culture technique.",
keywords = "anaerobic culture technique, real-time PCR, periodontitis, SUBGINGIVAL PLAQUE SAMPLES, PORPHYROMONAS-GINGIVALIS, ACTINOBACILLUS-ACTINOMYCETEMCOMITANS, UNITED-STATES, RISK FACTOR, DNA PROBE, DISEASE, BACTERIA, CULTURE, QUANTIFICATION",
author = "K Boutaga and {Van Winkelhoff}, AJ and CMJE Vandenbroucke-Grauls and PHM Savelkoul",
year = "2006",
month = jun,
doi = "10.1111/j.1600-051X.2006.00925.x",
language = "English",
volume = "33",
pages = "427--433",
journal = "Journal of Clinical Periodontology",
issn = "0303-6979",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - The additional value of real-time PCR in the quantitative detection of periodontal pathogens

AU - Boutaga, K

AU - Van Winkelhoff, AJ

AU - Vandenbroucke-Grauls, CMJE

AU - Savelkoul, PHM

PY - 2006/6

Y1 - 2006/6

N2 - Background and Aim: For the analysis of subgingival plaque, anaerobic bacterial culture has been the gold standard for many years. Currently, molecular microbial techniques have become available to identify and quantify target organisms with high specificity and sensitivity. The technique of real-time polymerase chain reaction (RT-PCR) provides a new tool to detect oral pathogens both in oral and non-oral human infections. The aim of this study was to compare the RT-PCR and anaerobic culture for detection and quantification of six periodontal pathogens in periodontal health and disease.Material and Methods: Subgingival plaque samples from 259 adult patients with periodontitis and 111 healthy controls were analysed with quantitative anaerobic culture and quantitative RT-PCR for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Micromonas micros and Fusobacterium spp.Results: All species were more frequently isolated from patients than controls with both culture and RT-PCR. P. gingivalis, T. forsythia and M. micros appeared significant markers for disease with both techniques. P. intermedia was significantly associated with periodontitis by RT-PCR only (OR 9.7), whereas A. actinomycetemcomitans showed a significant relationship by culture only. The critical differences between culture and RT-PCR were culture-negative/PCR-positive samples which amounted to 7% for A. actinomycetemcomitans, 3% for P. gingivalis, 7% for T. forsythia, 20% for P. intermedia, 6% for M. micros, and 0.8% for Fusobacterium spp. in periodontitis patients and 12%, 3%, 2%, 35%, 14% and 0%, respectively, in the periodontally healthy group. Furthermore, periodontitis individuals had significantly higher amount of all of the test species in the subgingival plaque samples compared with healthy subjects.Conclusion: RT-PCR provides a new rapid diagnostic tool and opens the opportunity to detect small numbers of oral pathogens in clinical specimens, which are under the detection limit by culture technique.

AB - Background and Aim: For the analysis of subgingival plaque, anaerobic bacterial culture has been the gold standard for many years. Currently, molecular microbial techniques have become available to identify and quantify target organisms with high specificity and sensitivity. The technique of real-time polymerase chain reaction (RT-PCR) provides a new tool to detect oral pathogens both in oral and non-oral human infections. The aim of this study was to compare the RT-PCR and anaerobic culture for detection and quantification of six periodontal pathogens in periodontal health and disease.Material and Methods: Subgingival plaque samples from 259 adult patients with periodontitis and 111 healthy controls were analysed with quantitative anaerobic culture and quantitative RT-PCR for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Micromonas micros and Fusobacterium spp.Results: All species were more frequently isolated from patients than controls with both culture and RT-PCR. P. gingivalis, T. forsythia and M. micros appeared significant markers for disease with both techniques. P. intermedia was significantly associated with periodontitis by RT-PCR only (OR 9.7), whereas A. actinomycetemcomitans showed a significant relationship by culture only. The critical differences between culture and RT-PCR were culture-negative/PCR-positive samples which amounted to 7% for A. actinomycetemcomitans, 3% for P. gingivalis, 7% for T. forsythia, 20% for P. intermedia, 6% for M. micros, and 0.8% for Fusobacterium spp. in periodontitis patients and 12%, 3%, 2%, 35%, 14% and 0%, respectively, in the periodontally healthy group. Furthermore, periodontitis individuals had significantly higher amount of all of the test species in the subgingival plaque samples compared with healthy subjects.Conclusion: RT-PCR provides a new rapid diagnostic tool and opens the opportunity to detect small numbers of oral pathogens in clinical specimens, which are under the detection limit by culture technique.

KW - anaerobic culture technique

KW - real-time PCR

KW - periodontitis

KW - SUBGINGIVAL PLAQUE SAMPLES

KW - PORPHYROMONAS-GINGIVALIS

KW - ACTINOBACILLUS-ACTINOMYCETEMCOMITANS

KW - UNITED-STATES

KW - RISK FACTOR

KW - DNA PROBE

KW - DISEASE

KW - BACTERIA

KW - CULTURE

KW - QUANTIFICATION

U2 - 10.1111/j.1600-051X.2006.00925.x

DO - 10.1111/j.1600-051X.2006.00925.x

M3 - Article

VL - 33

SP - 427

EP - 433

JO - Journal of Clinical Periodontology

JF - Journal of Clinical Periodontology

SN - 0303-6979

IS - 6

ER -

ID: 13926739