Publication

The 2018 correlative microscopy techniques roadmap

Ando, T., Bhamidimarri, S. P., Brending, N., Colin-York, H., Collinson, L., De Jonge, N., de Pablo, P. J., Debroye, E., Eggeling, C., Franck, C., Fritzsche, M., Gerritsen, H., Giepmans, B. N. G., Grunewald, K., Hofkens, J., Hoogenboom, J. P., Janssen, K. P. F., Kaufman, R., Klumpermann, J., Kurniawan, N., Kusch, J., Liv, N., Parekh, V., Peckys, D. B., Rehfeldt, F., Reutens, D. C., Roeffaers, M. B. J., Salditt, T., Schaap, I. A. T., Schwarz, U. S., Verkade, P., Vogel, M. W., Wagner, R., Winterhalter, M., Yuan, H. & Zifarelli, G., 7-Nov-2018, In : Journal of Physics D-Applied Physics. 51, 44, 42 p., 443001.

Research output: Contribution to journalReview articleAcademicpeer-review

APA

Ando, T., Bhamidimarri, S. P., Brending, N., Colin-York, H., Collinson, L., De Jonge, N., ... Zifarelli, G. (2018). The 2018 correlative microscopy techniques roadmap. Journal of Physics D-Applied Physics, 51(44), [443001]. https://doi.org/10.1088/1361-6463/aad055

Author

Ando, Toshio ; Bhamidimarri, Satya Prathyusha ; Brending, Niklas ; Colin-York, H. ; Collinson, Lucy ; De Jonge, Niels ; de Pablo, P. J. ; Debroye, Elke ; Eggeling, Christian ; Franck, Christian ; Fritzsche, Marco ; Gerritsen, Hans ; Giepmans, Ben N. G. ; Grunewald, Kay ; Hofkens, Johan ; Hoogenboom, Jacob P. ; Janssen, Kris P. F. ; Kaufman, Rainer ; Klumpermann, Judith ; Kurniawan, Nyoman ; Kusch, Jana ; Liv, Nalan ; Parekh, Viha ; Peckys, Diana B. ; Rehfeldt, Florian ; Reutens, David C. ; Roeffaers, Maarten B. J. ; Salditt, Tim ; Schaap, Iwan A. T. ; Schwarz, Ulrich S. ; Verkade, Paul ; Vogel, Michael W. ; Wagner, Richard ; Winterhalter, Mathias ; Yuan, Haifeng ; Zifarelli, Giovanni. / The 2018 correlative microscopy techniques roadmap. In: Journal of Physics D-Applied Physics. 2018 ; Vol. 51, No. 44.

Harvard

Ando, T, Bhamidimarri, SP, Brending, N, Colin-York, H, Collinson, L, De Jonge, N, de Pablo, PJ, Debroye, E, Eggeling, C, Franck, C, Fritzsche, M, Gerritsen, H, Giepmans, BNG, Grunewald, K, Hofkens, J, Hoogenboom, JP, Janssen, KPF, Kaufman, R, Klumpermann, J, Kurniawan, N, Kusch, J, Liv, N, Parekh, V, Peckys, DB, Rehfeldt, F, Reutens, DC, Roeffaers, MBJ, Salditt, T, Schaap, IAT, Schwarz, US, Verkade, P, Vogel, MW, Wagner, R, Winterhalter, M, Yuan, H & Zifarelli, G 2018, 'The 2018 correlative microscopy techniques roadmap', Journal of Physics D-Applied Physics, vol. 51, no. 44, 443001. https://doi.org/10.1088/1361-6463/aad055

Standard

The 2018 correlative microscopy techniques roadmap. / Ando, Toshio; Bhamidimarri, Satya Prathyusha; Brending, Niklas; Colin-York, H.; Collinson, Lucy; De Jonge, Niels; de Pablo, P. J.; Debroye, Elke; Eggeling, Christian; Franck, Christian; Fritzsche, Marco; Gerritsen, Hans; Giepmans, Ben N. G.; Grunewald, Kay; Hofkens, Johan; Hoogenboom, Jacob P.; Janssen, Kris P. F.; Kaufman, Rainer; Klumpermann, Judith; Kurniawan, Nyoman; Kusch, Jana; Liv, Nalan; Parekh, Viha; Peckys, Diana B.; Rehfeldt, Florian; Reutens, David C.; Roeffaers, Maarten B. J.; Salditt, Tim; Schaap, Iwan A. T.; Schwarz, Ulrich S.; Verkade, Paul; Vogel, Michael W.; Wagner, Richard; Winterhalter, Mathias; Yuan, Haifeng; Zifarelli, Giovanni.

In: Journal of Physics D-Applied Physics, Vol. 51, No. 44, 443001, 07.11.2018.

Research output: Contribution to journalReview articleAcademicpeer-review

Vancouver

Ando T, Bhamidimarri SP, Brending N, Colin-York H, Collinson L, De Jonge N et al. The 2018 correlative microscopy techniques roadmap. Journal of Physics D-Applied Physics. 2018 Nov 7;51(44). 443001. https://doi.org/10.1088/1361-6463/aad055


BibTeX

@article{35ce99102bb64072a7284413d3a897fc,
title = "The 2018 correlative microscopy techniques roadmap",
abstract = "Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x-ray scattering and multiple super-resolution fluorescence techniques, and each of these methods provides valuable read-outs to meet the demands set by the samples under study. Yet, the investigation of cell development requires a multi-parametric approach to address both the structure and spatio-temporal organization of organelles, and also the transduction of chemical signals and forces involved in cell-cell interactions. Although the microscopy technologies for observing each of these characteristics are well developed, none of them can offer read-out of all characteristics simultaneously, which limits the information content of a measurement. For example, while electron microscopy is able to disclose the structural layout of cells and the macromolecular arrangement of proteins, it cannot directly follow dynamics in living cells. The latter can be achieved with fluorescence microscopy which, however, requires labelling and lacks spatial resolution. A remedy is to combine and correlate different readouts from the same specimen, which opens new avenues to understand structure-function relations in biomedical research. At the same time, such correlative approaches pose new challenges concerning sample preparation, instrument stability, region of interest retrieval, and data analysis. Because the field of correlative microscopy is relatively young, the capabilities of the various approaches have yet to be fully explored, and uncertainties remain when considering the best choice of strategy and workflow for the correlative experiment. With this in mind, the Journal of Physics D: Applied Physics presents a special roadmap on the correlative microscopy techniques, giving a comprehensive overview from various leading scientists in this field, via a collection of multiple short viewpoints.",
keywords = "correlative microscopy, fluorescence microscopy, x-ray microscopy, electron microscopy, magnetic resonance imaging, atomic force microscopy, super-resolution microscopy, SCANNING-ELECTRON-MICROSCOPY, ATOMIC-FORCE MICROSCOPY, HIGH-RESOLUTION, SUPERRESOLUTION FLUORESCENCE, INTEGRATED LIGHT, LOCALIZATION MICROSCOPY, ENDOGENOUS PROTEINS, OPTICAL MICROSCOPY, MEMBRANE-PROTEINS, LIVING CELLS",
author = "Toshio Ando and Bhamidimarri, {Satya Prathyusha} and Niklas Brending and H. Colin-York and Lucy Collinson and {De Jonge}, Niels and {de Pablo}, {P. J.} and Elke Debroye and Christian Eggeling and Christian Franck and Marco Fritzsche and Hans Gerritsen and Giepmans, {Ben N. G.} and Kay Grunewald and Johan Hofkens and Hoogenboom, {Jacob P.} and Janssen, {Kris P. F.} and Rainer Kaufman and Judith Klumpermann and Nyoman Kurniawan and Jana Kusch and Nalan Liv and Viha Parekh and Peckys, {Diana B.} and Florian Rehfeldt and Reutens, {David C.} and Roeffaers, {Maarten B. J.} and Tim Salditt and Schaap, {Iwan A. T.} and Schwarz, {Ulrich S.} and Paul Verkade and Vogel, {Michael W.} and Richard Wagner and Mathias Winterhalter and Haifeng Yuan and Giovanni Zifarelli",
year = "2018",
month = "11",
day = "7",
doi = "10.1088/1361-6463/aad055",
language = "English",
volume = "51",
journal = "Journal of Physics D-Applied Physics",
issn = "0022-3727",
publisher = "IOP PUBLISHING LTD",
number = "44",

}

RIS

TY - JOUR

T1 - The 2018 correlative microscopy techniques roadmap

AU - Ando, Toshio

AU - Bhamidimarri, Satya Prathyusha

AU - Brending, Niklas

AU - Colin-York, H.

AU - Collinson, Lucy

AU - De Jonge, Niels

AU - de Pablo, P. J.

AU - Debroye, Elke

AU - Eggeling, Christian

AU - Franck, Christian

AU - Fritzsche, Marco

AU - Gerritsen, Hans

AU - Giepmans, Ben N. G.

AU - Grunewald, Kay

AU - Hofkens, Johan

AU - Hoogenboom, Jacob P.

AU - Janssen, Kris P. F.

AU - Kaufman, Rainer

AU - Klumpermann, Judith

AU - Kurniawan, Nyoman

AU - Kusch, Jana

AU - Liv, Nalan

AU - Parekh, Viha

AU - Peckys, Diana B.

AU - Rehfeldt, Florian

AU - Reutens, David C.

AU - Roeffaers, Maarten B. J.

AU - Salditt, Tim

AU - Schaap, Iwan A. T.

AU - Schwarz, Ulrich S.

AU - Verkade, Paul

AU - Vogel, Michael W.

AU - Wagner, Richard

AU - Winterhalter, Mathias

AU - Yuan, Haifeng

AU - Zifarelli, Giovanni

PY - 2018/11/7

Y1 - 2018/11/7

N2 - Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x-ray scattering and multiple super-resolution fluorescence techniques, and each of these methods provides valuable read-outs to meet the demands set by the samples under study. Yet, the investigation of cell development requires a multi-parametric approach to address both the structure and spatio-temporal organization of organelles, and also the transduction of chemical signals and forces involved in cell-cell interactions. Although the microscopy technologies for observing each of these characteristics are well developed, none of them can offer read-out of all characteristics simultaneously, which limits the information content of a measurement. For example, while electron microscopy is able to disclose the structural layout of cells and the macromolecular arrangement of proteins, it cannot directly follow dynamics in living cells. The latter can be achieved with fluorescence microscopy which, however, requires labelling and lacks spatial resolution. A remedy is to combine and correlate different readouts from the same specimen, which opens new avenues to understand structure-function relations in biomedical research. At the same time, such correlative approaches pose new challenges concerning sample preparation, instrument stability, region of interest retrieval, and data analysis. Because the field of correlative microscopy is relatively young, the capabilities of the various approaches have yet to be fully explored, and uncertainties remain when considering the best choice of strategy and workflow for the correlative experiment. With this in mind, the Journal of Physics D: Applied Physics presents a special roadmap on the correlative microscopy techniques, giving a comprehensive overview from various leading scientists in this field, via a collection of multiple short viewpoints.

AB - Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x-ray scattering and multiple super-resolution fluorescence techniques, and each of these methods provides valuable read-outs to meet the demands set by the samples under study. Yet, the investigation of cell development requires a multi-parametric approach to address both the structure and spatio-temporal organization of organelles, and also the transduction of chemical signals and forces involved in cell-cell interactions. Although the microscopy technologies for observing each of these characteristics are well developed, none of them can offer read-out of all characteristics simultaneously, which limits the information content of a measurement. For example, while electron microscopy is able to disclose the structural layout of cells and the macromolecular arrangement of proteins, it cannot directly follow dynamics in living cells. The latter can be achieved with fluorescence microscopy which, however, requires labelling and lacks spatial resolution. A remedy is to combine and correlate different readouts from the same specimen, which opens new avenues to understand structure-function relations in biomedical research. At the same time, such correlative approaches pose new challenges concerning sample preparation, instrument stability, region of interest retrieval, and data analysis. Because the field of correlative microscopy is relatively young, the capabilities of the various approaches have yet to be fully explored, and uncertainties remain when considering the best choice of strategy and workflow for the correlative experiment. With this in mind, the Journal of Physics D: Applied Physics presents a special roadmap on the correlative microscopy techniques, giving a comprehensive overview from various leading scientists in this field, via a collection of multiple short viewpoints.

KW - correlative microscopy

KW - fluorescence microscopy

KW - x-ray microscopy

KW - electron microscopy

KW - magnetic resonance imaging

KW - atomic force microscopy

KW - super-resolution microscopy

KW - SCANNING-ELECTRON-MICROSCOPY

KW - ATOMIC-FORCE MICROSCOPY

KW - HIGH-RESOLUTION

KW - SUPERRESOLUTION FLUORESCENCE

KW - INTEGRATED LIGHT

KW - LOCALIZATION MICROSCOPY

KW - ENDOGENOUS PROTEINS

KW - OPTICAL MICROSCOPY

KW - MEMBRANE-PROTEINS

KW - LIVING CELLS

U2 - 10.1088/1361-6463/aad055

DO - 10.1088/1361-6463/aad055

M3 - Review article

VL - 51

JO - Journal of Physics D-Applied Physics

T2 - Journal of Physics D-Applied Physics

JF - Journal of Physics D-Applied Physics

SN - 0022-3727

IS - 44

M1 - 443001

ER -

ID: 64813185