Publication

Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

Colpa, D. I., Zhou, W., Wempe, J. P., Tamis, J., Stuart, M. C. A., Krooneman, J. & Euverink, G-J. W., 21-Feb-2020, In : AIMS Bioengineering. 7, 1, 14 p., 19.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Colpa, D. I., Zhou, W., Wempe, J. P., Tamis, J., Stuart, M. C. A., Krooneman, J., & Euverink, G-J. W. (2020). Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium. AIMS Bioengineering, 7(1), [19]. https://doi.org/10.3390/bioengineering7010019

Author

Colpa, Dana I ; Zhou, Wen ; Wempe, Jan Pier ; Tamis, Jelmer ; Stuart, Marc C A ; Krooneman, Janneke ; Euverink, Gert-Jan W. / Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium. In: AIMS Bioengineering. 2020 ; Vol. 7, No. 1.

Harvard

Colpa, DI, Zhou, W, Wempe, JP, Tamis, J, Stuart, MCA, Krooneman, J & Euverink, G-JW 2020, 'Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium', AIMS Bioengineering, vol. 7, no. 1, 19. https://doi.org/10.3390/bioengineering7010019

Standard

Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium. / Colpa, Dana I; Zhou, Wen; Wempe, Jan Pier; Tamis, Jelmer; Stuart, Marc C A; Krooneman, Janneke; Euverink, Gert-Jan W.

In: AIMS Bioengineering, Vol. 7, No. 1, 19, 21.02.2020.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Colpa DI, Zhou W, Wempe JP, Tamis J, Stuart MCA, Krooneman J et al. Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium. AIMS Bioengineering. 2020 Feb 21;7(1). 19. https://doi.org/10.3390/bioengineering7010019


BibTeX

@article{d1c0796b6f3d428dae76a797bb002d88,
title = "Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium",
abstract = "Polyhydroxyalkanoates (PHAs) form a highly promising class of bioplastics for the transition from fossil fuel-based plastics to bio-renewable and biodegradable plastics. Mixed microbial consortia (MMC) are known to be able to produce PHAs from organic waste streams. Knowledge of key-microbes and their characteristics in PHA-producing consortia is necessary for further process optimization and direction towards synthesis of specific types of PHAs. In this study, a PHA-producing mixed microbial consortium (MMC) from an industrial pilot plant was characterized and further enriched on acetate in a laboratory-scale selector with a working volume of 5 L, and 16S-rDNA microbiological population analysis of both the industrial pilot plant and the 5 L selector revealed that the most dominant species within the population is Thauera aminoaromatica MZ1T, a Gram-negative beta-proteobacterium belonging to the order of the Rhodocyclales. The relative abundance of this Thauera species increased from 24 to 40% after two months of enrichment in the selector-system, indicating a competitive advantage, possibly due to the storage of a reserve material such as PHA. First experiments with T. aminoaromatica MZ1T showed multiple intracellular granules when grown in pure culture on a growth medium with a C:N ratio of 10:1 and acetate as a carbon source. Nuclear magnetic resonance (NMR) analyses upon extraction of PHA from the pure culture confirmed polyhydroxybutyrate production by T. aminoaromatica MZ1T.",
keywords = "Thauera aminoaromatica MZ1T, biopolymers, polyhydroxyalkanoate (PHA), polyhydroxybutyrate (PHB), 16S-rDNA analysis, biodiversity, mixed microbial consortium",
author = "Colpa, {Dana I} and Wen Zhou and Wempe, {Jan Pier} and Jelmer Tamis and Stuart, {Marc C A} and Janneke Krooneman and Euverink, {Gert-Jan W}",
year = "2020",
month = feb,
day = "21",
doi = "10.3390/bioengineering7010019",
language = "English",
volume = "7",
journal = "AIMS Bioengineering",
issn = "2306-5354",
publisher = "AIMS Press",
number = "1",

}

RIS

TY - JOUR

T1 - Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

AU - Colpa, Dana I

AU - Zhou, Wen

AU - Wempe, Jan Pier

AU - Tamis, Jelmer

AU - Stuart, Marc C A

AU - Krooneman, Janneke

AU - Euverink, Gert-Jan W

PY - 2020/2/21

Y1 - 2020/2/21

N2 - Polyhydroxyalkanoates (PHAs) form a highly promising class of bioplastics for the transition from fossil fuel-based plastics to bio-renewable and biodegradable plastics. Mixed microbial consortia (MMC) are known to be able to produce PHAs from organic waste streams. Knowledge of key-microbes and their characteristics in PHA-producing consortia is necessary for further process optimization and direction towards synthesis of specific types of PHAs. In this study, a PHA-producing mixed microbial consortium (MMC) from an industrial pilot plant was characterized and further enriched on acetate in a laboratory-scale selector with a working volume of 5 L, and 16S-rDNA microbiological population analysis of both the industrial pilot plant and the 5 L selector revealed that the most dominant species within the population is Thauera aminoaromatica MZ1T, a Gram-negative beta-proteobacterium belonging to the order of the Rhodocyclales. The relative abundance of this Thauera species increased from 24 to 40% after two months of enrichment in the selector-system, indicating a competitive advantage, possibly due to the storage of a reserve material such as PHA. First experiments with T. aminoaromatica MZ1T showed multiple intracellular granules when grown in pure culture on a growth medium with a C:N ratio of 10:1 and acetate as a carbon source. Nuclear magnetic resonance (NMR) analyses upon extraction of PHA from the pure culture confirmed polyhydroxybutyrate production by T. aminoaromatica MZ1T.

AB - Polyhydroxyalkanoates (PHAs) form a highly promising class of bioplastics for the transition from fossil fuel-based plastics to bio-renewable and biodegradable plastics. Mixed microbial consortia (MMC) are known to be able to produce PHAs from organic waste streams. Knowledge of key-microbes and their characteristics in PHA-producing consortia is necessary for further process optimization and direction towards synthesis of specific types of PHAs. In this study, a PHA-producing mixed microbial consortium (MMC) from an industrial pilot plant was characterized and further enriched on acetate in a laboratory-scale selector with a working volume of 5 L, and 16S-rDNA microbiological population analysis of both the industrial pilot plant and the 5 L selector revealed that the most dominant species within the population is Thauera aminoaromatica MZ1T, a Gram-negative beta-proteobacterium belonging to the order of the Rhodocyclales. The relative abundance of this Thauera species increased from 24 to 40% after two months of enrichment in the selector-system, indicating a competitive advantage, possibly due to the storage of a reserve material such as PHA. First experiments with T. aminoaromatica MZ1T showed multiple intracellular granules when grown in pure culture on a growth medium with a C:N ratio of 10:1 and acetate as a carbon source. Nuclear magnetic resonance (NMR) analyses upon extraction of PHA from the pure culture confirmed polyhydroxybutyrate production by T. aminoaromatica MZ1T.

KW - Thauera aminoaromatica MZ1T

KW - biopolymers

KW - polyhydroxyalkanoate (PHA)

KW - polyhydroxybutyrate (PHB)

KW - 16S-rDNA analysis

KW - biodiversity

KW - mixed microbial consortium

U2 - 10.3390/bioengineering7010019

DO - 10.3390/bioengineering7010019

M3 - Article

C2 - 32098069

VL - 7

JO - AIMS Bioengineering

JF - AIMS Bioengineering

SN - 2306-5354

IS - 1

M1 - 19

ER -

ID: 119307390