Publication

Telomere length homeostasis responds to changes in intracellular dNTP pools

Gupta, A., Sharma, S., Reichenbach, P., Marjavaara, L., Nilsson, A. K., Lingner, J., Chabes, A., Rothstein, R. & Chang, M., Apr-2013, In : Genetics. 193, 4, p. 1095-1105 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Gupta, A., Sharma, S., Reichenbach, P., Marjavaara, L., Nilsson, A. K., Lingner, J., Chabes, A., Rothstein, R., & Chang, M. (2013). Telomere length homeostasis responds to changes in intracellular dNTP pools. Genetics, 193(4), 1095-1105. https://doi.org/10.1534/genetics.112.149120

Author

Gupta, Amitabha ; Sharma, Sushma ; Reichenbach, Patrick ; Marjavaara, Lisette ; Nilsson, Anna Karin ; Lingner, Joachim ; Chabes, Andrei ; Rothstein, Rodney ; Chang, Michael. / Telomere length homeostasis responds to changes in intracellular dNTP pools. In: Genetics. 2013 ; Vol. 193, No. 4. pp. 1095-1105.

Harvard

Gupta, A, Sharma, S, Reichenbach, P, Marjavaara, L, Nilsson, AK, Lingner, J, Chabes, A, Rothstein, R & Chang, M 2013, 'Telomere length homeostasis responds to changes in intracellular dNTP pools', Genetics, vol. 193, no. 4, pp. 1095-1105. https://doi.org/10.1534/genetics.112.149120

Standard

Telomere length homeostasis responds to changes in intracellular dNTP pools. / Gupta, Amitabha; Sharma, Sushma; Reichenbach, Patrick; Marjavaara, Lisette; Nilsson, Anna Karin; Lingner, Joachim; Chabes, Andrei; Rothstein, Rodney; Chang, Michael.

In: Genetics, Vol. 193, No. 4, 04.2013, p. 1095-1105.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Gupta A, Sharma S, Reichenbach P, Marjavaara L, Nilsson AK, Lingner J et al. Telomere length homeostasis responds to changes in intracellular dNTP pools. Genetics. 2013 Apr;193(4):1095-1105. https://doi.org/10.1534/genetics.112.149120


BibTeX

@article{063a04e6a26640dab39a5485885959b3,
title = "Telomere length homeostasis responds to changes in intracellular dNTP pools",
abstract = "Telomeres, the ends of linear eukaryotic chromosomes, shorten due to incomplete DNA replication and nucleolytic degradation. Cells counteract this shortening by employing a specialized reverse transcriptase called telomerase, which uses deoxyribonucleoside triphosphates (dNTPs) to extend telomeres. Intracellular dNTP levels are tightly regulated, and perturbation of these levels is known to affect DNA synthesis. We examined whether altering the levels of the dNTP pools or changing the relative ratios of the four dNTPs in Saccharomyces cerevisiae would affect the length of the telomeres. Lowering dNTP levels leads to a modest shortening of telomeres, while increasing dNTP pools has no significant effect on telomere length. Strikingly, altering the ratio of the four dNTPs dramatically affects telomere length homeostasis, both positively and negatively. Specifically, we find that intracellular deoxyguanosine triphosphate (dGTP) levels positively correlate with both telomere length and telomerase nucleotide addition processivity in vivo. Our findings are consistent with in vitro data showing dGTP-dependent stimulation of telomerase activity in multiple organisms and suggest that telomerase activity is modulated in vivo by dGTP levels.",
keywords = "REPEAT ADDITION PROCESSIVITY, S-PHASE CHECKPOINT, SACCHAROMYCES-CEREVISIAE, RIBONUCLEOTIDE REDUCTASE, DNA-DAMAGE, TETRAHYMENA-TELOMERASE, CELL-CYCLE, REVERSE-TRANSCRIPTASE, EUPLOTES TELOMERASE, CATALYTIC SUBUNIT",
author = "Amitabha Gupta and Sushma Sharma and Patrick Reichenbach and Lisette Marjavaara and Nilsson, {Anna Karin} and Joachim Lingner and Andrei Chabes and Rodney Rothstein and Michael Chang",
year = "2013",
month = apr,
doi = "10.1534/genetics.112.149120",
language = "English",
volume = "193",
pages = "1095--1105",
journal = "Genetics",
issn = "0016-6731",
publisher = "GENETICS SOCIETY AMERICA",
number = "4",

}

RIS

TY - JOUR

T1 - Telomere length homeostasis responds to changes in intracellular dNTP pools

AU - Gupta, Amitabha

AU - Sharma, Sushma

AU - Reichenbach, Patrick

AU - Marjavaara, Lisette

AU - Nilsson, Anna Karin

AU - Lingner, Joachim

AU - Chabes, Andrei

AU - Rothstein, Rodney

AU - Chang, Michael

PY - 2013/4

Y1 - 2013/4

N2 - Telomeres, the ends of linear eukaryotic chromosomes, shorten due to incomplete DNA replication and nucleolytic degradation. Cells counteract this shortening by employing a specialized reverse transcriptase called telomerase, which uses deoxyribonucleoside triphosphates (dNTPs) to extend telomeres. Intracellular dNTP levels are tightly regulated, and perturbation of these levels is known to affect DNA synthesis. We examined whether altering the levels of the dNTP pools or changing the relative ratios of the four dNTPs in Saccharomyces cerevisiae would affect the length of the telomeres. Lowering dNTP levels leads to a modest shortening of telomeres, while increasing dNTP pools has no significant effect on telomere length. Strikingly, altering the ratio of the four dNTPs dramatically affects telomere length homeostasis, both positively and negatively. Specifically, we find that intracellular deoxyguanosine triphosphate (dGTP) levels positively correlate with both telomere length and telomerase nucleotide addition processivity in vivo. Our findings are consistent with in vitro data showing dGTP-dependent stimulation of telomerase activity in multiple organisms and suggest that telomerase activity is modulated in vivo by dGTP levels.

AB - Telomeres, the ends of linear eukaryotic chromosomes, shorten due to incomplete DNA replication and nucleolytic degradation. Cells counteract this shortening by employing a specialized reverse transcriptase called telomerase, which uses deoxyribonucleoside triphosphates (dNTPs) to extend telomeres. Intracellular dNTP levels are tightly regulated, and perturbation of these levels is known to affect DNA synthesis. We examined whether altering the levels of the dNTP pools or changing the relative ratios of the four dNTPs in Saccharomyces cerevisiae would affect the length of the telomeres. Lowering dNTP levels leads to a modest shortening of telomeres, while increasing dNTP pools has no significant effect on telomere length. Strikingly, altering the ratio of the four dNTPs dramatically affects telomere length homeostasis, both positively and negatively. Specifically, we find that intracellular deoxyguanosine triphosphate (dGTP) levels positively correlate with both telomere length and telomerase nucleotide addition processivity in vivo. Our findings are consistent with in vitro data showing dGTP-dependent stimulation of telomerase activity in multiple organisms and suggest that telomerase activity is modulated in vivo by dGTP levels.

KW - REPEAT ADDITION PROCESSIVITY

KW - S-PHASE CHECKPOINT

KW - SACCHAROMYCES-CEREVISIAE

KW - RIBONUCLEOTIDE REDUCTASE

KW - DNA-DAMAGE

KW - TETRAHYMENA-TELOMERASE

KW - CELL-CYCLE

KW - REVERSE-TRANSCRIPTASE

KW - EUPLOTES TELOMERASE

KW - CATALYTIC SUBUNIT

U2 - 10.1534/genetics.112.149120

DO - 10.1534/genetics.112.149120

M3 - Article

C2 - 23335335

VL - 193

SP - 1095

EP - 1105

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 4

ER -

ID: 5832924