Publication

Telomerase regulation by the Pif1 helicase: a length-dependent effect?

Stinus, S., Paeschke, K. & Chang, M., Apr-2018, In : Current Genetics. 64, 2, p. 509-513 5 p.

Research output: Contribution to journalArticleAcademicpeer-review

Dysfunctional telomere length regulation is detrimental to human health, and both activation and inhibition of telomerase have been proposed in potential therapies to treat human diseases. The Saccharomyces cerevisiae Pif1 protein is an evolutionarily conserved helicase that inhibits telomerase activity at DNA ends. Recent studies have indicated that Pif1 is specifically important for inhibiting telomerase at DNA ends with very little or no telomeric sequence and at long telomeres. At the former, Pif1 prevents the inappropriate addition of a telomere at DNA double-strand breaks. For the latter, Pif1 has been shown to bind long telomeres to presumably promote the extension of the short ones. These observations leave the impression that Pif1 does not act at DNA ends with telomeric sequence of intermediate length. Here, we provide in vivo evidence that Pif1 inhibits telomerase activity at DNA ends regardless of telomere sequence length.

Original languageEnglish
Pages (from-to)509-513
Number of pages5
JournalCurrent Genetics
Volume64
Issue number2
Early online date20-Oct-2017
Publication statusPublished - Apr-2018

    Keywords

  • Pif1, Telomeres, Telomerase, Telomere length homeostasis, Double-strand breaks, SACCHAROMYCES-CEREVISIAE, DNA-REPLICATION, YEAST, CANCER, PROTEIN, BREAKS, ENDS

View graph of relations

Download statistics

No data available

ID: 51655591