Publication

Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer

Galli, F., Manni, I., Piaggio, G., Balogh, L., Weintraub, B. D., Szkudlinski, M. W., Fremont, V., Dierckx, R. A. J. O. & Signore, A., 2014, In : Thyroid. 24, 8, p. 1297-1308 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Galli, F., Manni, I., Piaggio, G., Balogh, L., Weintraub, B. D., Szkudlinski, M. W., ... Signore, A. (2014). Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer. Thyroid, 24(8), 1297-1308. https://doi.org/10.1089/thy.2013.0429

Author

Galli, Filippo ; Manni, Isabella ; Piaggio, Giulia ; Balogh, Lajos ; Weintraub, Bruce D. ; Szkudlinski, Mariusz W. ; Fremont, Valerie ; Dierckx, Rudi A. J. O. ; Signore, Alberto. / Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer. In: Thyroid. 2014 ; Vol. 24, No. 8. pp. 1297-1308.

Harvard

Galli, F, Manni, I, Piaggio, G, Balogh, L, Weintraub, BD, Szkudlinski, MW, Fremont, V, Dierckx, RAJO & Signore, A 2014, 'Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer', Thyroid, vol. 24, no. 8, pp. 1297-1308. https://doi.org/10.1089/thy.2013.0429

Standard

Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer. / Galli, Filippo; Manni, Isabella; Piaggio, Giulia; Balogh, Lajos; Weintraub, Bruce D.; Szkudlinski, Mariusz W.; Fremont, Valerie; Dierckx, Rudi A. J. O.; Signore, Alberto.

In: Thyroid, Vol. 24, No. 8, 2014, p. 1297-1308.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Galli F, Manni I, Piaggio G, Balogh L, Weintraub BD, Szkudlinski MW et al. Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer. Thyroid. 2014;24(8):1297-1308. https://doi.org/10.1089/thy.2013.0429


BibTeX

@article{8ac78c68cc4c42db93c80cb35138da96,
title = "Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer",
abstract = "Background: Differentiated thyroid carcinomas originating from thyroid follicular cells are frequent tumors of the thyroid with relatively good prognosis due to improved surgical techniques and follow-up procedures. Poorly differentiated thyroid cancers, which lose iodine uptake ability, in most cases still express thyrotropin (TSH) receptors (TSHR). Therefore, the aim of this study was to radiolabel a superagonist recombinant human TSH (rhTSH) analogue for imaging poorly differentiated thyroid cancer.Methods: The TSHR superagonist, TR1401, was labeled with Tc-99m using an indirect method via succinimidyl-6-hydrazinonicotinate hydrochloride conjugation. In vitro quality controls included SDS-PAGE, cysteine challenge, and cell-binding assay on TSHR positive cell lines (JP09 and ML-1). In vivo studies included tumor targeting experiments in athymic nude CD-1 mice xenografted with several different TSHR positive cells (JP09, K1, and ML-1) and TSHR negative cells (JP02) as control.Results: The superagonist rhTSH analogue TR1401 was labeled with high labeling efficiency (>95{\%}) and high specific activity (9250 MBq/mg). The labeled molecule retained its biologic activity and structural integrity. In tumor targeting experiments, a focal uptake of radiolabeled TR1401 was observed in TSHR positive cells but not in TSHR negative cells. The same observation was made in a dog with spontaneous intraglandular thyroid cancer.Conclusions: We were able to radiolabel the rhTSH superagonist analogue TR1401 with Tc-99m efficiently with retention of in vitro and in vivo binding capacity to TSHR. The relative role of such novel radiopharmaceutical versus I-131 scanning of thyroid cancer will require future histopathologic and clinical studies, but it may open new perspectives for presurgical staging of thyroid cancer, and diagnosis of radioiodine negative local relapses and/or distant metastases.",
keywords = "RECOMBINANT HUMAN TSH, STIMULATING HORMONE, CELL-LINES, THYROTROPIN STIMULATION, SUPERACTIVE ANALOGS, DIAGNOSTIC-VALUE, I-124 PET/CT, IN-VIVO, CARCINOMA, RECEPTOR",
author = "Filippo Galli and Isabella Manni and Giulia Piaggio and Lajos Balogh and Weintraub, {Bruce D.} and Szkudlinski, {Mariusz W.} and Valerie Fremont and Dierckx, {Rudi A. J. O.} and Alberto Signore",
year = "2014",
doi = "10.1089/thy.2013.0429",
language = "English",
volume = "24",
pages = "1297--1308",
journal = "Thyroid",
issn = "1050-7256",
publisher = "MARY ANN LIEBERT, INC",
number = "8",

}

RIS

TY - JOUR

T1 - Tc-99m-Labeled-rhTSH Analogue (TR1401) for Imaging Poorly Differentiated Metastatic Thyroid Cancer

AU - Galli, Filippo

AU - Manni, Isabella

AU - Piaggio, Giulia

AU - Balogh, Lajos

AU - Weintraub, Bruce D.

AU - Szkudlinski, Mariusz W.

AU - Fremont, Valerie

AU - Dierckx, Rudi A. J. O.

AU - Signore, Alberto

PY - 2014

Y1 - 2014

N2 - Background: Differentiated thyroid carcinomas originating from thyroid follicular cells are frequent tumors of the thyroid with relatively good prognosis due to improved surgical techniques and follow-up procedures. Poorly differentiated thyroid cancers, which lose iodine uptake ability, in most cases still express thyrotropin (TSH) receptors (TSHR). Therefore, the aim of this study was to radiolabel a superagonist recombinant human TSH (rhTSH) analogue for imaging poorly differentiated thyroid cancer.Methods: The TSHR superagonist, TR1401, was labeled with Tc-99m using an indirect method via succinimidyl-6-hydrazinonicotinate hydrochloride conjugation. In vitro quality controls included SDS-PAGE, cysteine challenge, and cell-binding assay on TSHR positive cell lines (JP09 and ML-1). In vivo studies included tumor targeting experiments in athymic nude CD-1 mice xenografted with several different TSHR positive cells (JP09, K1, and ML-1) and TSHR negative cells (JP02) as control.Results: The superagonist rhTSH analogue TR1401 was labeled with high labeling efficiency (>95%) and high specific activity (9250 MBq/mg). The labeled molecule retained its biologic activity and structural integrity. In tumor targeting experiments, a focal uptake of radiolabeled TR1401 was observed in TSHR positive cells but not in TSHR negative cells. The same observation was made in a dog with spontaneous intraglandular thyroid cancer.Conclusions: We were able to radiolabel the rhTSH superagonist analogue TR1401 with Tc-99m efficiently with retention of in vitro and in vivo binding capacity to TSHR. The relative role of such novel radiopharmaceutical versus I-131 scanning of thyroid cancer will require future histopathologic and clinical studies, but it may open new perspectives for presurgical staging of thyroid cancer, and diagnosis of radioiodine negative local relapses and/or distant metastases.

AB - Background: Differentiated thyroid carcinomas originating from thyroid follicular cells are frequent tumors of the thyroid with relatively good prognosis due to improved surgical techniques and follow-up procedures. Poorly differentiated thyroid cancers, which lose iodine uptake ability, in most cases still express thyrotropin (TSH) receptors (TSHR). Therefore, the aim of this study was to radiolabel a superagonist recombinant human TSH (rhTSH) analogue for imaging poorly differentiated thyroid cancer.Methods: The TSHR superagonist, TR1401, was labeled with Tc-99m using an indirect method via succinimidyl-6-hydrazinonicotinate hydrochloride conjugation. In vitro quality controls included SDS-PAGE, cysteine challenge, and cell-binding assay on TSHR positive cell lines (JP09 and ML-1). In vivo studies included tumor targeting experiments in athymic nude CD-1 mice xenografted with several different TSHR positive cells (JP09, K1, and ML-1) and TSHR negative cells (JP02) as control.Results: The superagonist rhTSH analogue TR1401 was labeled with high labeling efficiency (>95%) and high specific activity (9250 MBq/mg). The labeled molecule retained its biologic activity and structural integrity. In tumor targeting experiments, a focal uptake of radiolabeled TR1401 was observed in TSHR positive cells but not in TSHR negative cells. The same observation was made in a dog with spontaneous intraglandular thyroid cancer.Conclusions: We were able to radiolabel the rhTSH superagonist analogue TR1401 with Tc-99m efficiently with retention of in vitro and in vivo binding capacity to TSHR. The relative role of such novel radiopharmaceutical versus I-131 scanning of thyroid cancer will require future histopathologic and clinical studies, but it may open new perspectives for presurgical staging of thyroid cancer, and diagnosis of radioiodine negative local relapses and/or distant metastases.

KW - RECOMBINANT HUMAN TSH

KW - STIMULATING HORMONE

KW - CELL-LINES

KW - THYROTROPIN STIMULATION

KW - SUPERACTIVE ANALOGS

KW - DIAGNOSTIC-VALUE

KW - I-124 PET/CT

KW - IN-VIVO

KW - CARCINOMA

KW - RECEPTOR

U2 - 10.1089/thy.2013.0429

DO - 10.1089/thy.2013.0429

M3 - Article

VL - 24

SP - 1297

EP - 1308

JO - Thyroid

JF - Thyroid

SN - 1050-7256

IS - 8

ER -

ID: 16069926