Publication

Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Lohse, J., Schindl, A., Danda, N., Williams, C. P., Kramer, K., Kuster, B., Witte, M. D. & Médard, G., 11-Nov-2017, In : Chem. Commun.. 53, 87, p. 11929-11932 4 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Lohse, J., Schindl, A., Danda, N., Williams, C. P., Kramer, K., Kuster, B., ... Médard, G. (2017). Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy. Chem. Commun., 53(87), 11929-11932. https://doi.org/10.1039/C7CC07001B

Author

Lohse, Jonas ; Schindl, Alexandra ; Danda, Natasha ; Williams, Chris P ; Kramer, Karl ; Kuster, Bernhard ; Witte, Martin D ; Médard, Guillaume. / Target and identify : triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy. In: Chem. Commun. 2017 ; Vol. 53, No. 87. pp. 11929-11932.

Harvard

Lohse, J, Schindl, A, Danda, N, Williams, CP, Kramer, K, Kuster, B, Witte, MD & Médard, G 2017, 'Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy', Chem. Commun., vol. 53, no. 87, pp. 11929-11932. https://doi.org/10.1039/C7CC07001B

Standard

Target and identify : triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy. / Lohse, Jonas; Schindl, Alexandra; Danda, Natasha; Williams, Chris P; Kramer, Karl; Kuster, Bernhard; Witte, Martin D; Médard, Guillaume.

In: Chem. Commun., Vol. 53, No. 87, 11.11.2017, p. 11929-11932.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Lohse J, Schindl A, Danda N, Williams CP, Kramer K, Kuster B et al. Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy. Chem. Commun. 2017 Nov 11;53(87):11929-11932. https://doi.org/10.1039/C7CC07001B


BibTeX

@article{c353f980d0c849ba8bd9a56ee5ffda9d,
title = "Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy",
abstract = "A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.",
keywords = "AZIDE-ALKYNE CYCLOADDITION, CHEMICAL PROTEOMICS, BIOORTHOGONAL CHEMISTRY, BIOTIN LIGASE, ENRICHMENT, BIOCONJUGATION, REPORTERS, MIXTURES, RELEASE, BIOLOGY",
author = "Jonas Lohse and Alexandra Schindl and Natasha Danda and Williams, {Chris P} and Karl Kramer and Bernhard Kuster and Witte, {Martin D} and Guillaume M{\'e}dard",
year = "2017",
month = "11",
day = "11",
doi = "10.1039/C7CC07001B",
language = "English",
volume = "53",
pages = "11929--11932",
journal = "Chemical Communications (Cambridge, United Kingdom)",
issn = "1359-7345",
publisher = "ROYAL SOC CHEMISTRY",
number = "87",

}

RIS

TY - JOUR

T1 - Target and identify

T2 - triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

AU - Lohse, Jonas

AU - Schindl, Alexandra

AU - Danda, Natasha

AU - Williams, Chris P

AU - Kramer, Karl

AU - Kuster, Bernhard

AU - Witte, Martin D

AU - Médard, Guillaume

PY - 2017/11/11

Y1 - 2017/11/11

N2 - A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

AB - A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

KW - AZIDE-ALKYNE CYCLOADDITION

KW - CHEMICAL PROTEOMICS

KW - BIOORTHOGONAL CHEMISTRY

KW - BIOTIN LIGASE

KW - ENRICHMENT

KW - BIOCONJUGATION

KW - REPORTERS

KW - MIXTURES

KW - RELEASE

KW - BIOLOGY

U2 - 10.1039/C7CC07001B

DO - 10.1039/C7CC07001B

M3 - Article

VL - 53

SP - 11929

EP - 11932

JO - Chemical Communications (Cambridge, United Kingdom)

JF - Chemical Communications (Cambridge, United Kingdom)

SN - 1359-7345

IS - 87

ER -

ID: 49136584