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Target and identify: triazene linker helps identify azidation sites of labelled proteins via click and cleave strategy

Lohse, J., Schindl, A., Danda, N., Williams, C. P., Kramer, K., Kuster, B., Witte, M. D. & Médard, G., 11-Nov-2017, In : Chem. Commun.. 53, 87, p. 11929-11932 4 p.

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  • Target and identify

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DOI

  • Jonas Lohse
  • Alexandra Schindl
  • Natasha Danda
  • Chris P Williams
  • Karl Kramer
  • Bernhard Kuster
  • Martin D Witte
  • Guillaume Médard

A method for identifying probe modification of proteins via tandem mass spectrometry was developed. Azide bearing molecules are immobilized on functionalised sepharose beads via copper catalysed Huisgen-type click chemistry and selectively released under acidic conditions by chemical cleavage of the triazene linkage. We applied this method to identify the modification site of targeted-diazotransfer on BirA.

Original languageEnglish
Pages (from-to)11929-11932
Number of pages4
JournalChem. Commun.
Volume53
Issue number87
Publication statusPublished - 11-Nov-2017

    Keywords

  • AZIDE-ALKYNE CYCLOADDITION, CHEMICAL PROTEOMICS, BIOORTHOGONAL CHEMISTRY, BIOTIN LIGASE, ENRICHMENT, BIOCONJUGATION, REPORTERS, MIXTURES, RELEASE, BIOLOGY

ID: 49136584